Hilton 2010 J Comp Physiol B

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Hilton Z, Clements KD, Hickey AJ (2010) Temperature sensitivity of cardiac mitochondria in intertidal and subtidal triplefin fishes. J Comp Physiol B 180:979-90.

» PMID: 20461387

Hilton Z, Clements KD, Hickey AJ (2010) J Comp Physiol B

Abstract: The heart is acutely sensitive to temperature in aquatic ectotherms and appears to fail before any other organ as the thermal maximum is reached, although the exact cause of this failure remains unknown. The heart is highly aerobic and therefore dependent on mitochondrial oxidative phosphorylation (OXPHOS) to meet energy requirements, but the role of cardiac mitochondria in limiting heart function at high temperatures remains unclear. We used permeabilised ventricle fibres to explore heart mitochondrial function in situ in three closely related species of small New Zealand triplefin fishes in response to temperature. We compared this to measures of whole animal respiration rates and critical oxygen tensions in these fishes. Bellapiscis medius, an intertidal species, had the greatest tolerance to hypoxia at higher temperatures and had more efficient OXPHOS at 30 °C than the two subtidal species Forsterygion varium and F. malcolmi. B. medius also displayed the highest cytochrome c oxidase flux, which may in part explain how B. medius tolerates higher temperatures and hypoxia. Triplefin heart mitochondria exhibit decreased coupling to phosphorylation with increasing temperature. This most likely impairs ATP supply to the heart at elevated temperatures, potentially contributing to heart failure at ecologically relevant temperatures.

Keywords: Subtidal triplefin fishes, Bellapiscis medius, Forsterygion varium, Forsterygion malcolmi

O2k-Network Lab: NZ Auckland Hickey AJ


Labels: MiParea: Respiration, Comparative MiP;environmental MiP 


Organism: Fishes  Tissue;cell: Heart  Preparation: Permeabilized tissue 

Regulation: Cyt c, Substrate, Temperature  Coupling state: LEAK, OXPHOS, ET  Pathway: CIV, NS, ROX  HRR: Oxygraph-2k