Hoeks 2008 FEBS Lett

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Hoeks J, Briedé JJ, de Vogel J, Schaart G, Nabben M, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) Mitochondrial function, content and ROS production in rat skeletal muscle: Effect of high-fat feeding. FEBS Lett 582:510-16.

» PMID: 18230360 Open Access

Hoeks J, Briede JJ, de Vogel J, Schaart G, Nabben M, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) FEBS Lett

Abstract: A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respirometry, neither on a carbohydrate- nor on a lipid-substrate. Interestingly, PPARGC1A protein increased by approximately 2-fold upon high-fat feeding and we observed inconsistent results on different markers of mitochondrial density. Mitochondrial ROS production, assessed by electron spin resonance spectroscopy remained unaffected. Intramyocellular lipid levels increased significantly illustrating that a reduced innate mitochondrial function is not a prerequisite for intra-muscular fat accumulation.

Keywords: PGC-1α

O2k-Network Lab: NL Maastricht Schrauwen P


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style  Pathology: Obesity  Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 

Regulation: Substrate, Fatty acid  Coupling state: ROUTINE, OXPHOS, ET  Pathway: F, NS, ROX  HRR: Oxygraph-2k