Horseradish peroxidase

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Horseradish peroxidase

Description

Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.

Abbreviation: HRP

Application in HRR

HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C.
Preparation of 500 U/mL stock solution (dissolved in MiR05):
  1. Weigh 3,33 mg
  2. Dissolve in 1 mL MiR05.
  3. Aliquot 200 µL into 500 µL Eppendorf tubes.
The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Measurement of H2O2 with Amplex UltraRed


Reaction

Donor + H2O2 → Oxidized Donor + 2 H2O

Known inhibitors

According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.


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References

  1. Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.

MitoPedia methods: Fluorometry