MiPNet23.02 IOC127 Szeged HU

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MiPNet23.02 IOC127 Szeged HU

Publications in the MiPMap

Szeged HU, 2018 Mar 27. Oroboros satellite O2k-Workshop on hypoxia IOC127.

Oroboros (2018-03-27) Mitochondr Physiol Network



Oroboros O2k-Workshop on hypoxia in cooperation with HU Szeged Boros M. Szeged, Hungary, 2018 March 27.

O2k-Network Lab: AT_Innsbruck_OROBOROS, HU Szeged Boros M

General information

This O2k-Workshop takes place satellite to the WISe3 Conference in Szeged, HU. Separate registration is required for this workshop.
Programme overview
Detailed programme
Abstract book
Three topics will be covered during the one-day workshop:
(1) Andras Meszaros: Closed-chamber mitochondrial oxygen kinetics with demo experiment and introduction of the data analysis module O2kinetics - new development.
(2) Erich Gnaiger: Assessment of mitochondrial and cellular oxygen kinetics by steady-state oxygen titrations, using the Titration-Injection microPump TIP2k,
(3) Timea Komlodi: Measurement of H2O2 production rate with the O2k-FluoRespirometer and Amplex UltraRed(R) fluorescence. Dependence of ROS production on oxygen concentration.


Clinical Skills Centre, Faculty of Medicine
University of Szeged
13 Dom square, HU-6720
Szeged, Hungary
HU Szeged Boros M

Local organizers


The registration is free of charge, but we ask you for informal registration by Mar 19 at latest.
The number of participants will be limited. Please let us know by email if you will attend: andras.meszaros@oroboros.at

Tutors and Lecturers


Tuesday Mar 27

Time Topic Materials
09:00 Morning session on mitochondria
09:00 Petr Pecina Petr Pecina

Changes in oxygen kinetics between COX4i1 and COX4i2-containing COX

09:30 Laszlo Tretter Laszlo Tretter

Bioenergetical approach of neurodegenerative diseases

10:00 Laszlo Juhasz Laszlo Juhasz

Ca(2+)N it be measured? High-resolution O2k-FluoRespirometric detection of extramitochondrial calcium movement

10:10 David Kurszan Jasz

Mitochondrial effects of methane gas treatment on rat cardiomyocytes subjected to simulated ischemia/reperfusion

10:20 Eszter Tuboly

Novel cardioprotective effects of methane in an ex vivo model

10:30 Coffee/tea break
10:45 Andras Meszaros Andras Meszaros

Mitochondrial oxygen kinetics in closed-chamber respirometers - Introduction.

Gnaiger 1995 J Bioenerg Biomembr

Mitochondrial oxygen kinetics in closed-chamber respirometers - O2k demo experiment.


Mitochondrial oxygen kinetics in closed-chamber respirometers - Data analysis.

Introduction of the new DatLab analysis module O2kinetics

12:15 Lunch break
13:00 Chinopoulos Christos Chinopoulos Christos

Interplay of respiratory components and mitochondrial diaphorases on redox state in anoxia

13:30 Timea Komlodi Timea Komlodi

Oxygen dependence of H2O2 production: an application of high-resolution respirometry with Amplex UltraRed(R) fluorescence.

Hydrogen peroxide
DatLab Oxygen dependence of H2O2 production: Data analysis Hydrogen peroxide
14:30 Coffee/tea break
Erich Gnaiger
Erich Gnaiger

Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - A comparison of steady-state and closed-chamber mitochondrial oxygen kinetics.


Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Programming the TIP2k.


Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Calculations and data analysis. Theoretical background.

Harrison 2015 J Appl Physiol
16:15 Coffee/tea break
16:30 COST Action MitoEAGLE session
16:30 Ledo Ana Margarida

Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer’s disease: implications on mitochondrial function

16:45 Nina Krako Krako Jakovljević Nina

Investigation of subcellular mechanisms in insulin resistance models in hepatocytes and myocytes

17:00 Beata Cizmarova Beata Cizmarova

The effect of short-term exopsure to moderate altitude on respiration of peripheral-blood mononuclear cells

17:15 Poster session and discussions
Andrea Evinova

Magnesium protects from calcium induced collapse of mitochondrial trans-membrane potential.

Kasja Pavlovic Kasja Pavlovic

C2C12 myoblasts as a cell model for studying the role of mitochondria in insulin resistance

Ioana Pavel

The acute and chronic effects of a benzylamide derivative of maslinic acid in liver mitochondria isolated from mice with chemically induced skin carcinogenesis

List of participants

Participant Institution
Bori Zoltan University of Physical Education, Budapest
Budai Andras HU Budapest Szijjarto A - Semmelweis University
Chinopoulos Christos Chinopoulos Christos HU Budapest Chinopoulos C - Semmelweis University
Kolonics Attila Kolonics Attila BioFirmware Ltd
Koltai Erika University of Physical Education, Budapest
Pavel Ioana RO Timisoara Muntean DM - Victor Babes University of Medicine and Pharmacy
Pecina Petr Pecina Petr CZ Prague Houstek J - Academy of Sciences of the Czech Republic
Talas David University of Szeged
Sergeeva Tatiana RU Nizhny Novgorod Mukhina I - Nizhny Novgorod State Medical Academy
Tretter Laszlo Tretter Laszlo HU Budapest Tretter L - Semmelweis University
Windedal Tobias

WISe3 Conference Szeged

» WISe3 Conference


Oroboros Instruments
High-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

IOC recommended reading

» IOC recommended reading
Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«
  • Isolated mitochondria and intact cells.
  • Nanomolar resolution of oxygen concentration.
  • Effects of cell density and activity.
  • Closed versus open systems.
  • p50 in vivo.
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
  • A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen
  • Mitochondrial kinetics measured by high-resolution respirometry
  • Calibrations and corrections for response time and instrumental background
  • Steady-state injection respirometry
  • Mitochondrial respiratory control at low oxygen
  • Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
  • Effect of ADP and oxygen limitation on ADP/O2 flux ratios
  • The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«
  • First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph.
  • Standardized oxygen calibration (air and internal zero oxygen calibration).
  • Automatic instrumental background correction of oxygen flux.
  • Deconvolution of the oxygen signal for time resolution required in oxygen kinetics.
  • Quality control of the limit of detection of oxygen flux at ±1 pmol O2·s-1·ml-1.


HRR: Oxygraph-2k 

ORO, IOC, O2k-Network, 2018 

O2k-Workshops are listed as MitoGlobal Events.'
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