MiPNet23.11 IOC137 Innsbruck AT
 Innsbruck AT, 2018 Dec 10-12. MitoFit Coaching Days - Basic IOC137. |
Oroboros (2018-12-10) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Dec 10-12.
โข O2k-Network Lab: AT_Innsbruck_Oroboros
General information
- MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
- ยป Information on travel and venue: IOC Innsbruck.
- Please bring your labcoat with you. Thank you!
Feedback
- 11 feedback forms returned
- Instructors - so patient! So kind! Really good at explaining things over and over again.
- Thank you to all involved including other participants who were also kind and keen to learn and share.
- Great company and team!
- 11 feedback forms returned
| Question | Rating (average) |
|---|---|
| In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). | 4.27 |
| Balance between practical โhands-onโ work and โtheoreticalโ explanations: Please rate from 1 (too much โhands-onโ) to 5 (too much theory). | 3 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: | 4.18 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: | 4.36 |
| Level of tutoring during the โhands-onโ work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). | 3.14 |
| Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). | 4.82 |
| Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). | 2.77 |
| Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). | 4.32 |
Registration
- closed
Tutors, lecturers, and managers
Manuela Passrugger, BSc., Oroboros Instruments - Biomedical Assistant
Marco di Marcello, BSc., Medical University of Innsbruck, MitoFit project - Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments - PhD. student TRANSMIT
Garcia-Souza Luiz F, MSc., Leopold-Franzens-University - PhD. student
Carolina Doerrier, PhD., Oroboros Instruments - Chief scientific officer
Erich Gnaiger, PhD., Oroboros Instruments - CEO
Programme
Monday 10th Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests ORO-MitoFit Lab 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab 09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab 10:10 Hands-on: DatLab 7 ORO-MitoFit Lab 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
ORO-MitoFit Lab 13:30 Instrumental quality control 2: Instrumental O2 background โ overview with videoclips ORO-MitoFit Lab 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 11th Topic Room 09:00 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 09:15 Application of the O2k Series H ORO-MitoFit Lab 09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols โ fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:30 Introduction to DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Wednesday 12th Topic Room 09:00 MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 10:00 Coffee 10:30 MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:15 DatLab analysis ORO-MitoFit Lab 14:00 DatLab analysis summary ORO-MitoFit Lab 15:00 Coffee 15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt 16:15 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
| Participant | Institution | |
|---|---|---|
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Barlow Jonathan | UK Birmingham Lai YC, University of Birmingham, UK |
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Chakrabarti Lisa | UK Nottingham Chakrabarti L, University of Nottingham, UK |
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Gnaiger Carolina | AT Innsbruck Oroboros, Innsbruck, AT |
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Ho Dieu Hien | DE Cologne Trifunovic A, Institute of Mitocondiral Disease and Ageing, Cologne, DE |
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Jedlicka Jan | CZ Pilsen Kuncova J, Charles University, Plzne, CZ |
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Kapferer Werner | AT Innsbruck Jansen-Duerr P, University of Innsbruck, AT |
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Kranewitter Sabine | AT Innsbruck Oroboros, Innsbruck, AT |
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Maciej Sarah | DE Cologne Trifunovic A, Institute of Mitocondiral Disease and Ageing, Cologne, DE |
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Maseko Tumisang Edward | CZ Hradec Kralove Cervinkova Z, Charles University, Hradec Kralove, CZ |
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Oliveira Ben | UK Leicester Watson E, Kings College Hospital, London, UK |
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Saharnaz Sarlak | FR Bordeaux Rossignol R, University of Bordeaux, Bordeaux, FR |
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Thapa Maheshwor | AT Salzburg Sperl W, Biocrates Life Sciences AG, Innsbruck, AT |
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Ton Riccardo | AU Sydney Griffith SC, Macquarie University, Sydney, AU |
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Zdrazilova Lucie | CZ Prague Zeman J, Charles University, Prague, CZ |
Impressions of the IOC137
Group picture of IOC137 in front of the Oroboros Office
Group picture of IOC137 participants and tutors
Manuela Passrugger and Ana Carolina Bastos Sant'Anna Silva assisting while mounting membranes
Snapshot of some IOC137 participants during Marco di Marcello's presentation
Ho Dieu Hien, Oliveira Ben, Ton Riccardo and Maciej Sarah working with the O2k
Participants in action
Kapferer Werner and Barlow Jonathan running the experiment with Luiz Felipe Garcia-Souza's support
Snapshot of Maseko Tumisang Edward
Saharnaz Sarlak studying the material hand-outs
Manuela Passrugger assisting the Oroboros-Team members Gnaiger Carolina and Sabine Kranewitter during the experiment
Carolina Doerrier and Ton Riccardo after the successful experiment
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schรถpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, 2018, MitoFit, TRANSMIT, MitoGlobal
