Schroecken AT, 2011 Dec 10-15. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC65.
Abstract: O2k-Workshop on High-Resolution Respirometry: O2k-Basic and TPP-Basic. Schröcken, Vorarlberg, Austria; 2011 10 - 15 December
ORO, IOC, 2011
Introductory Workshop in Parallel Sessions: O2k Basic and TPP Basic
The workshop is run in parallel sessions: O2k Basic and TPP Basic. Specific emphasis is placed on hands-on practical sessions with the instrument and the software DatLab 4.3, to introduce the participants into the technical and operational details of the method. The workshop programme is complemented by combined lectures (general topics; substrate-uncoupler-inhibitor titration SUIT protocols) and experiments run in "Special interest groups". Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects. Please register for O2k Basic or TPP Basic according to your specific needs and interest.
- O2k Basic includes a basic introduction to the Oroboros O2k, overview presentations on high resolution respirometry (HRR), and applications in mitochondrial physiology.
- TPP Basic is customized particularly for users interested in MultiSensor applications, particularly ion selective electrodes for measurement of mitochondrial membrane potential (TPP+, TPMP+). It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) will constitue a major part. According to interest of the participant other MultiSensor applications, like measurement of Ca2+, calibration and calculation of free Ca2+ in buffered solutions and acidification rate (pH) can be discussed/demonstrated. Please indicate your topics of interest (Ca2+, pH etc.) in advance.
Lecturers and tutors
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
Fasching Mario, O2k-Network Lab: Oroboros Instruments
Meissner Barbara, O2k-Network Lab: Oroboros Instruments
- Draxl Anna, O2k-Network Lab: Oroboros Instruments
|Bechara-Hirvonen Nada||University of Helsinki (FI)||Mitochondrial dysfunction in experimental heart failure.|
|Bienholz Anja||DE Essen Bienholz A: Universitätsklinikum Essen (DE)||Role of non-esterified fatty acids in respiratory impairment and mitochondrialde-energization of proximal tubules secondary to hypoxia/reoxygenation.|
|Bosch Marta||ES Barcelona Bosch M: IDIBAPS, Hospital Clínic (ES)|
|Cannon Megan||Experimental Cardiology UMCG (NL)||Metabolic remodeling in cardiac hypertrophy and heart failure.|
|Cesnekova Jana||CZ Prague Zeman J: Charles University (CZ)||Oxidative phosphorylation system.|
|Cotter Matthew||US AR Little Rock Borsheim E: University of Arkansas for Medical Sciences (US)||Changes in skeletal muscle respiration in burned children.|
|Farsijani Navid||US TN Nashville Wasserman DH: Vanderbilt University Medical Center (US)||High resolution respirometry of mouse kidney tissue.|
|Ghosh Sangeeta A||US TX San Antonio Musi N: University Center for Community Health (US)||Effect of aging and exercise training on mitochondrial function: relationship with insulin sensitivity and inflammation. Present work is focused on the study of signaling mechanisms that mediate insulin resistance in aging skeletal muscle, focusing mainly on mitochondrial function and its relationship with inflammatory signaling pathways.|
|Goncalves Renata de Lima Sales||BR Rio de Janeiro Oliveira MF: Federal University of Rio de Janeiro (BR)||Mitochondrial membrane potential, metabolic states.|
|Gonzalez-Franquesa Alba||ES Barcelona Garcia-Roves PM: University of Barcelona (ES)||Mitochondrial dysfunction in insulin-dependent tissues such as liver, skeletal muscle and adipose tissue.|
|Hinkeldeim Tanja||Universitätsklinikum Essen (DE)||Respiration in isolated proximate tubules (kidney) after hypoxia/ reoxygenation supported by different cac metabolites.|
|Hoffman David L||US MI Ann Arbor Glick GD: University of Michigan (US)||Mitochondrial ROS production / O2; open flow respirometry mitochondrial therapeutics.|
|Keipert Susanne||DE Nuthetal Klaus S: German Institute of Human Nutrition (DE)||Mitochondrial uncoupling in skeletal muscle: Transgenic mice with ectopic expression of UCP1 in skeletal muscle with beneficial effects on energy metabolism, diet-induced obesity and aging.|
|Kerätär Juha||Department of Biochemistry (FI)||Effect of mitochondrial fatty acid synthesis defects on mitochondrial respiration.|
|Knaub Leslie||US CO Denver Schauer I: University Colorado Denver (US)||Mitochondrial dysfunction in the vasculature and skeletal muscle in disease states.|
|Lau Gigi||CA Vancouver Richards JG: The University of British Columbia (CA)||Physiological adaptations of mitochondria to hypoxia in fishes.|
|Lundby Carsten||CH Zurich Lundby C: University of Zurich (CH)|
|McDonald Allison E||Wilfrid Laurier University (CA)||Functional and regulatory characterization of alternative oxidases in various kingdoms of life.|
|Nielsen Morten Hostrup||SE Stockholm Boushel RC: The Swedish School of Sport and Health Sciences (SE)||Effects of beta2-agonists on human skeletal muscle mitochondrial enzyme activity.|
|Oliveira Marcus F||BR Rio de Janeiro Oliveira MF: Federal University of Rio de Janeiro (BR)||Studies on the mitochondrial metabolism in the blood flow Schistosoma mansoni.|
|Orynbayeva Zulfiya||US PA Philadelphia Orynbayeva Z: Drexel University College of Medicine (US)||The bioenergetic signature of cancer; the case of prostate adenocarcinoma.|
|Ost Mario||DE Nuthetal Klaus S: German Institute of Human Nutrition (DE)||Mitochondrial uncoupling in skeletal muscle: Transgenic mice with ectopic expression of UCP1 in skeletal muscle with beneficial effects on energy metabolism, diet-induced obesity and aging.|
|Plenge Ulla||SE Stockholm Boushel RC: The Swedish School of Sport and Health Sciences (SE)||Effects of rhEPO in human mitochondria in muscle cells and blood cells.|
|Pinho Joao Paulo||BR Rio de Janeiro Oliveira MF: Federal University of Rio de Janeiro (BR)||Studies on the dynamic changes of mitochondrial function following a blood meal in the kissing bug Rhodnius prolixus.|
|Hans Sondergard Nielsen||SE Stockholm Boushel RC||Regulation of mitochondria in different bioetnic groups. Imact og diet and exercise. Further the impact of klima and light, circadian rythm on mitochondria.|
|Krizova Jana||CZ Prague Zeman J: Charles University (CZ)||Oxidative metabolism in human skin fibroblast from patients with mitochondrial disorders.|
|Strand Harald||University Hospital North Norway||Diagnosis of mitochondrial diseases.|
Hot topics in mitochondrial physiology - abstracts
|Goncalves 2011 Abstract IOC65||Gonçalves RL, Barretoc RFSM, Polycarpod CR, Castro SL, Gadelhae FR, Oliveira MF (2011) Comparative assessment of mitochondrial function on epimastigotes and bloodstream trypomastigotes of Trypanosoma cruzi. MiPNet16.03.|
|Bienholz 2011 Abstract IOC65||Bienholz AH, Feldkamp T, Weinberg JM (2011) Role of Nonesterified Fatty Acids in Respiratory Impariment and Mitochondrial Deenergization of Proximal Renal Tubules Secondary to Hypoxia/Reoxygenation. MiPNet16.03.|
|Pinho 2011 Abstract IOC65||Pinho JPC, Goncalves RL, Oliveira MF (2011) Studies on the dynamic changes of mitochondrial function following a blood meal in the kissing bug Rhodnius prolixus. MiPNet16.03.|
|Ghosh 2011 Abstract IOC65||Ghosh SA, Lertwattanarak R, Richardson AG, Van Remmen H, DeFronzo RA, Musi N (2011) Effect of aging and exercise training on mitochondrial function in skeletal muscle. MiPNet16.03.|
|Oliveira 2011 Abstract IOC65||Oliveira MP, Goncalves RLS, Oliveira MF (2011) Studies on the mitochondrial metabolism in the blood fluke Schistosoma mansoni. MiPNet16.03.|
|McDonald 2011 Abstract IOC65||McDonald AE (2011) Alternative oxidase respiratory capacity in eukaryotes and prokaryotes. MiPNet16.03.|
|Panov 2011 Abstract IOC65||Panov A, Orynbayeva Z (2011) The bioenergetic signature of prostate cancer. MiPNet16.03.|
- We thank the participants for taking the time to fill in the 'Feedback forms' and answer our questions.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom Tyrol.