MiPNet19.05 IOC90 Bolzano IT

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MiPNet19.05 IOC90 Bolzano IT


Publications in the MiPMap
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Bolzano IT, 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. IOC90 at ISMM2014.

» Bioblast pdf

Oroboros (2014-05-27) Mitochondr Physiol Network

Abstract:
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90th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.



O2k-Network Lab: AT Innsbruck Oroboros


Labels:

Stress:Ischemia-reperfusion, Temperature 




HRR: Oxygraph-2k 

ORO, IOC, 2014, MitoCom 



IOC90-Bolzano.jpg

Conference

  • ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31

O2k-Workshop

TUESDAY 2014-05-27 / 14.00 - 17.30
Seminar room 8
The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the Oroboros O2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.
» More details? Consult Hypothermia-Network: Mitochondria in the Cold.

Introduction

14:00 – 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments


Erich Gnaiger Fontana-Ayoub Mona Fleischmann Sandra Laner Verena


Gnaiger Erich, CEO; Fontana-Ayoub Mona; Biomedical assistant; Fleischmann Sandra; Administrative assistant; Laner Verena; Project manager and scientific assistant

Demo experiment

14:45 – 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia

15:30 – 16:00 Coffee break

Round table: Hypoxia and mitochondrial function - practical experience

16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oroboros O2k to >4,000 m

Erich Gnaiger, Greenland Robert Boushel Adam Chicco Andrew Murray James Horscroft
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.

Round table: Hypothermia and mitochondrial function - perspectives

16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function

Keynote statements by
  • Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants
Edward Ojuka, Adam Chicco, Erich Gnaiger; ISMM2014 Bolzano

Participants

  • Harald Engan (Mid Sweden University)
  • Irene Pichler (EURAC Bolzano/Bozen - Center for Biomedicine)
  • Lindsay Benage (Colorado State University)
  • Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine)
  • Mark Olfert (West Virginia University)
  • Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin)
  • Chris Müller (LMU München)
  • Vittore Verrati (Chieti University)
  • Marika Falla (Ospedale di Bolzano, Neurologia)
  • Paolo Laveder (University of Padova, Italy, Dep of Biology)
  • Daniel Martin (University College London)
  • Edward Ojuka (University of Cape Town, South Africa)


Guest tutors and invited experts

  • Robert Boushel (The Swedish School of Sport and Health Sciences)
  • Adam Chicco (Colorado State University, Department of Health and Exercise Science)
  • Andrew Murray (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
  • James Horscroft (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
  • Sandro Malacrida (EURAC Bolzano/Bozen - Center for Biomedicine)


Registration

  • Visit us at the Oroboros Instruments exhibition booth from Monday to Wednesday, May 26 to 28.
  • Here you can register for the Oroboros O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.


Recommended reading


SUIT protocols for high-resolution respirometry

O2k-Publications in the MiPMap
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access


HRR and O2k-Fluorometry

» Manual: MiPNet17.05 O2k-Fluo LED2-Module
  • Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access
» O2k-Fluorometry


Mitochondrial pathways

O2k-Protocols
  • Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. » Open Access - handout to O2k-Workshop participants.


MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.
  • This workshop is a component of the K-Regio project MitoCom.