Jansen 2019 Am J Physiol Heart Circ Physiol
|Jansen KM, Moreno S, Garcia-Roves PM, Larsen TS (2019) Dietary Calanus oil recovers metabolic flexibility and rescues post-ischemic cardiac function in obese female mice. Am J Physiol Heart Circ Physiol [Epub ahead of print].|
Abstract: The aim of this study was to find out if dietary supplementation with Calanus oil (a novel marine oil) or infusion of exenatide (an incretin mimetic) can counteract obesity-induced alterations in myocardial metabolism and improve post-ischemic recovery of left ventricular (LV) function. Female C57bl/6J mice received high-fat diet (HFD, 45% energy from fat) for 12 weeks, followed by 8 weeks feeding with non-supplemented HFD, HFD supplemented with 2% Calanus oil, or HFD plus exenatide infusion (10 µg/kg/day). A lean control group was included, receiving normal chow throughout the whole period. Fatty acid and glucose oxidation was measured in ex vivo perfused hearts during baseline conditions, while LV function was assessed by an intra-ventricular fluid-filled balloon before and after 20 min global ischemia. HFD-fed mice receiving Calanus oil or exenatide showed less intra-abdominal fat deposition, compared to mice receiving non-supplemented HFD. Both treatments prevented the HFD-induced decline in myocardial glucose oxidation. Somewhat surprising, recovery of LV function was apparently better in hearts from mice fed non-supplemented HFD, relative to hearts from mice fed normal chow. More importantly however, post-ischemic recovery of hearts from mice receiving HFD with Calanus oil was superior to that of mice receiving non-supplemented HFD and mice receiving HFD with exenatide, as expressed by better pressure development, contractility and relaxation properties. In summary, dietary Calanus oil and administration of exenatide counteracted obesity-induced derangements of myocardial metabolism. Calanus oil also protected the heart towards ischemia, which could have implications for the prevention of obesity-related cardiac disease.
• Keywords: Ischemia-reperfusion, Myocardial fatty acid oxidation, Myocardial glucose oxidation, Obesity, Ventricular function • Bioblast editor: Plangger M • O2k-Network Lab: NO Tromsoe Larsen TS, ES Barcelona Garcia-Roves PM
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style Pathology: Obesity Stress:Ischemia-reperfusion Organism: Mouse Tissue;cell: Heart Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k