Juarez-Flores 2020 Antioxidants (Basel)

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Juarez-Flores DL, Ezquerra M, Gonzalez-Casacuberta I, Ormazabal A, Moren C, Tolosa E, Fucho R, Guitart-Mampel M, Casado M, Valldeoriola F, de la Torre-Lara J, Munoz E, Tobias E, Compta Y, Garcia-Garcia FJ, Garcia-Ruiz C, Fernandez-Checa JC, Marti MJ, Grau JM, Cardellach F, Artuch R, Fernandez-Santiago R, Garrabou G (2020) Disrupted mitochondrial and metabolic plasticity underlie comorbidity between age-related and degenerative disorders as Parkinson disease and type 2 diabetes mellitus. Antioxidants (Basel) 9:1063.

» PMID: 33143119 Open Access

Juarez-Flores Diana Luz, Ezquerra Mario, Gonzalez-Casacuberta Ingrid, Ormazabal Aida, Moren Constanza, Tolosa Eduardo, Fucho Raquel, Guitart-Mampel Mariona, Casado Mercedes, Valldeoriola Francesc, de la Torre-Lara Joan, Munoz Esteban, Tobias Ester, Compta Yaroslau, Garcia-Garcia Francesc Josep, Garcia-Ruiz Carmen, Fernandez-Checa Jose Carlos, Marti Maria Jose, Grau Josep Maria, Cardellach Francesc, Artuch Rafael, Fernandez-Santiago Ruben, Garrabou Gloria (2020) Antioxidants (Basel)

Abstract: Idiopathic Parkinson’s disease (iPD) and type 2 diabetes mellitus (T2DM) are chronic, multisystemic, and degenerative diseases associated with aging, with eventual epidemiological co-morbidity and overlap in molecular basis. This study aims to explore if metabolic and mitochondrial alterations underlie the previously reported epidemiologic and clinical co-morbidity from a molecular level.

To evaluate the adaptation of iPD to a simulated pre-diabetogenic state, we exposed primary cultured fibroblasts from iPD patients and controls to standard (5 mM) and high (25 mM) glucose concentrations to further characterize metabolic and mitochondrial resilience.

iPD fibroblasts showed increased organic and amino acid levels related to mitochondrial metabolism with respect to controls, and these differences were enhanced in high glucose conditions (citric, suberic, and sebacic acids levels increased, as well as alanine, glutamate, aspartate, arginine, and ornithine amino acids; p-values between 0.001 and 0.05). The accumulation of metabolites in iPD fibroblasts was associated with (and probably due to) the concomitant mitochondrial dysfunction observed at enzymatic, oxidative, respiratory, and morphologic level. Metabolic and mitochondrial plasticity of controls was not observed in iPD fibroblasts, which were unable to adapt to different glucose conditions. Impaired metabolism and mitochondrial activity in iPD may limit energy supply for cell survival. Moreover, reduced capacity to adapt to disrupted glucose balance characteristic of T2DM may underlay the co-morbidity between both diseases.

Fibroblasts from iPD patients showed mitochondrial impairment, resulting in the accumulation of organic and amino acids related to mitochondrial metabolism, especially when exposed to high glucose. Mitochondrial and metabolic defects down warding cell plasticity to adapt to changing glucose bioavailability may explain the comorbidity between iPD and T2DM. Keywords: T2DM (type 2 diabetes mellitus), iPD (idiopathic Parkinson’s disease), Metabolome, Mitochondria Bioblast editor: Reiswig R O2k-Network Lab: ES Barcelona Moren C


Labels: MiParea: Respiration, Patients  Pathology: Diabetes, Parkinson's 

Organism: Human  Tissue;cell: Fibroblast  Preparation: Permeabilized cells, Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2021-08