Kabiri 2021b Methods Mol Biol

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Kabiri Y, Eberhagen C, Schmitt S, Knolle PA, Zischka H (2021) Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria. Methods Mol Biol 2277:277-287.

» PMID:34080157

Kabiri Y, Eberhagen C, Schmitt S, Knolle PA, Zischka H (2021) Methods Mol Biol

Abstract: Isolation of mitochondria is a crucial method for examining molecular details of this organelle's manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for the subsequent analysis of such isolated mitochondria by electron microscopy. Combining the isolation procedure with this powerful imaging method can reveal ultrastructural mitochondrial peculiarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.

Cited by

  • Komlodi T et al (2021) Oxygen dependence of hydrogen peroxide production using Amplex UltraRed in yeast cells, isolated mitochondria, and permeabilized cells. MitoFit Preprints 2021 (in prep).

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