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Kahraman 2007 Neurochem Res

From Bioblast
Publications in the MiPMap
Kahraman S, Fiskum G (2007) Anoxia-induced changes in pyridine nucleotide redox state in cortical neurons and astrocytes. Neurochem Res 32:799-806.

ยป PMID: 17191134 Open Access

Kahraman S, Fiskum G (2007) Neurochem Res

Abstract: NAD(P)H autofluorescence was used to verify establishment of metabolic anoxia using primary cultures of cortical neurons and astrocytes. Cells on cover slips were placed in a chamber and O(2) was displaced by continuous infusion of argon. Perfusion with medium at PO(2) < 0.4 mm Hg caused an increase in NAD(P)H fluorescence, albeit to levels lower than that obtained with cyanide. Addition of the nitric oxide-generating agent DETA-NO to the hypoxic medium further increased fluorescence to the level with cyanide. Fluorescence under anoxia remained high in the presence of glucose, but declined in neurons and not in astrocytes when glucose was substituted with 2-deoxyglucose. Reoxygenation of neurons resulted in a decline in fluorescence and a loss in fluorescent gradient between fully reduced and fully oxidized (plus respiratory uncoupler). We conclude that (1) DETA-NO is useful for generating metabolic anoxia in the presence of argon (2) Exogenous glucose is necessary to maintain NAD(P)H in a reduced state during metabolic anoxia in neurons but not astrocytes (3) Neurons undergo a partially irreversible decline in NAD(P)H fluorescence during metabolic anoxia and reoxygenation that could contribute to prolonged metabolic failure. โ€ข Keywords: mitochondria, cytochrome oxidase, nitric oxide, fluorescence, respiration โ€ข Bioblast editor: Doerrier C


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