Kelly 2015 Biotechnol J

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Kelly PS, Breen L, Gallagher C, Kelly S, Henry M, Lao NT, Meleady P, O'Gorman D, Clynes M, Barron N (2015) Re-programming CHO cell metabolism using miR-23 tips the balance towards a highly productive phenotype. Biotechnol J 10:1029-40.

» PMID: 26097147

Kelly PS, Breen L, Gallagher C, Kelly S, Henry M, Lao NT, Meleady P, O'Gorman D, Clynes M, Barron N (2015) Biotechnol J

Abstract: microRNA engineering of CHO cells has already proved successful in enhancing various industrially relevant phenotypes and producing various recombinant products. A single miRNA's ability to interact with multiple mRNA targets allows their regulatory capacity to extend to processes such as cellular metabolism. Various metabolic states have previously been associated with particular CHO cell phenotypes such as glycolytic or oxidative metabolism accommodating growth and productivity, respectively. miR-23 has previously been demonstrated to play a role in glutamate metabolism resulting in enhanced oxidative phosphorylation through the TCA cycle. Re-programming cellular bioenergetics through miR-23 could tip the balance, forcing mammalian production cells to be more productive by favoring metabolic channelling into oxidative metabolism. CHO clones depleted of miR-23 using a miR-sponge decoy demonstrated an average ∼three-fold enhanced specific productivity with no impact on cell growth. Using a cell respirometer, mitochondrial activity was found to be enhanced by ∼30% at Complex I and II of the electron transport system. Additionally, label-free proteomic analysis uncovered various potential novel targets of miR-23 including LE™1 and IDH1, both implicated in oxidative metabolism and mitochondrial activity. These results demonstrate miRNA-based engineering as a route to re-programming cellular metabolism resulting in increased productivity, without affecting growth.

Keywords: Chinese hamster ovary (CHO), Mitochondria, Productivity, miR-23, microRNA-sponge

O2k-Network Lab: IE Dublin O Gorman D


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics 


Tissue;cell: CHO  Preparation: Intact cells, Permeabilized cells 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k