Khera 2015 Placenta

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Khera A, Dong LF, Holland OJ, Vanderlelie J, Pasdar EA, Neuzil J, Perkins AV (2015) Selenium supplementation induces mitochondrial biogenesis in trophoblasts. Placenta 36:863-9.

» PMID: 26154583

Khera A, Dong LF, Holland OJ, Vanderlelie J, Pasdar EA, Neuzil J, Perkins AV (2015) Placenta

Abstract: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium.

Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by Western blotting.

Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.

These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts.

Keywords: Selenium, Reactive oxygen species, Mitochondrial biogenesis, Antioxydant, Trophoblast, Swan-71 cells, Human placenta choriocarcinoma BeWo cells, Human choriocarcinoma JEG-3 cells

O2k-Network Lab: CZ Prague Neuzil J, AU Queensland Neuzil J, AU Queensland Peart J


Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics, Pharmacology;toxicology 


Organism: Human  Tissue;cell: Genital, Other cell lines  Preparation: Intact cells, Permeabilized cells 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, CIV  HRR: Oxygraph-2k