Khera 2015 Placenta
|Khera A, Dong LF, Holland OJ, Vanderlelie J, Pasdar EA, Neuzil J, Perkins AV (2015) Selenium supplementation induces mitochondrial biogenesis in trophoblasts. Placenta 36:863-9.|
Abstract: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium.
Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-1α and NRF-1 was examined by Western blotting.
Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells.
These results emphasise the importance of selenium in mitochondrial regeneration in trophoblasts.
• Keywords: Selenium, Reactive oxygen species, Mitochondrial biogenesis, Antioxydant, Trophoblast, Swan-71 cells, Human placenta choriocarcinoma BeWo cells, Human choriocarcinoma JEG-3 cells
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics, Pharmacology;toxicology
Organism: Human Tissue;cell: Genital, Other cell lines Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, S, CIV HRR: Oxygraph-2k