Kluckova 2015 Methods Mol Biol
|Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94.|
Abstract: Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.
• Keywords: Mitochondrial respiration, Cultured cells, Tumors, Mitochondria-targeted anticancer agents, Mouse mesothelioma tumor cells AE 17 cells
Labels: MiParea: Respiration, Instruments;methods, mt-Medicine, Pharmacology;toxicology Pathology: Cancer
Organism: Mouse Tissue;cell: Endothelial;epithelial;mesothelial cell, Genital, Other cell lines Preparation: Intact cells, Permeabilized cells, Homogenate
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k