Koenitzer 2007 Am J Physiol Heart Circ Physiol

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Koenitzer JR, Isbell TS, Patel HD, Benavides GA, Dickinson DA, Patel RP, Darley-Usmar VM, Lancaster JR Jr, Doeller JE, Kraus DW (2007) Hydrogen sulfide mediates vasoactivity in an O2-dependent manner. Am J Physiol Heart Circ Physiol 292:H1953-60.

» PMID: 17237242 Open Access

Koenitzer JR, Isbell TS, Patel HD, Benavides GA, Dickinson DA, Patel RP, Darley-Usmar VM, Lancaster JR Jr, Doeller JE, Kraus DW (2007) Am J Physiol Heart Circ Physiol

Abstract: Hydrogen sulfide (H2S) has recently been shown to have a signaling role in vascular cells. Similar to nitric oxide (NO), H2S is enzymatically produced by amino acid metabolism and can cause posttranslational modification of proteins, particularly at thiol residues. Molecular targets for H2S include ATP-sensitive K+ channels, and H2S may interact with NO and heme proteins such as cyclooxygenase. It is well known that the reactions of NO in the vasculature are O2 dependent, but this has not been addressed in most studies designed to elucidate the role of H2S in vascular function. This is important, since H2S reactions can be dramatically altered by the high concentrations of O2 used in cell culture and organ bath experiments. To test the hypothesis that the effects of H2S on the vasculature are O2 dependent, we have measured real-time levels of H2S and O2 in respirometry and vessel tension experiments, as well as the associated vascular responses. A novel polarographic H2S sensor developed in our laboratory was used to measure H2S levels. Here we report that, in rat aorta, H2S concentrations that mediate rapid contraction at high O2 levels cause rapid relaxation at lower physiological O2 levels. At high O2, the vasoconstrictive effect of H2S suggests that it may not be H2S per se but, rather, a putative vasoactive oxidation product that mediates constriction. These data are interpreted in terms of the potential for H2S to modulate vascular tone in vivo. Keywords: Aorta, Sulfide sensor, H2S, Vasorelaxation

O2k-Network Lab: US AL Birmingham Kraus DW


Labels:

Stress:Ischemia-reperfusion  Organism: Rat  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Intact organ  Enzyme: Inner mt-membrane transporter, Marker enzyme 

Coupling state: OXPHOS 

HRR: Oxygraph-2k