Kozlov 2010 Inflammation
|Kozlov AV, van Griensven M, Haindl S, Kehrer I, Duvigneau JC, Hartl RT, Ebel T, Jafarmadar M, Calzia E, Gnaiger E, Redl H, Radermacher P, Bahrami Sl (2010) Peritoneal inflammation in pigs is associated with early mitochondrial dysfunction in liver and kidney. Inflammation 33:295-305.|
Abstract: The objective of this study was to investigate early effects of peritoneal inflammation on the mitochondrial function in the vital organs, liver and kidney, and their relation to inflammatory and oxidative stress mediators. The study was performed on 14 domestic pigs. Peritoneal inflammation was induced in anesthetized pigs after a midline laparotomy by autologous feces. Fluid resuscitation maintained a MAP above 60 mmHg. Animals were sacrificed 12 h later, and tissue samples were obtained to determine mitochondrial function, mRNA levels of relevant genes [inducible NO synthase (iNOS), inducible HO (HO-1), tumor necrosis factor-alpha (TNF-alpha)], generation of reactive oxygen species (ROS), and HO-1 activity. We found impaired mitochondrial function in both liver and kidney, based on decreased State 3 respiration in the liver and increased States 2 and 4 respiration in the kidney at 12 h. This was accompanied by increased TNF-alpha protein in the blood and up-regulation of TNF-alpha mRNA in the liver. Free iron was elevated in the liver but not in the kidney. In the kidney, mitochondrial ROS production was increased. Nitric oxide levels in blood remained unchanged, corresponding to unchanged levels of iNOS mRNA expression in liver and kidney. Similarly, HO-1 mRNA and heme oxygenase (HO)-activity were unchanged. The inflammatory response in the absence of characteristic septic symptoms was not associated with morphological organ damage at this early time point. Peritoneal inflammation in pigs caused mitochondrial dysfunction in liver and kidney, preceding signs of organ damage. We did not find proof that mitochondrial dysfunction was due to increased levels of either nitric oxide (NO) or products of HO, but it was accompanied by increased levels of oxidative stress markers.
• Keywords: Inflammation, Pig, Mitochondria, Reactive oxygen and nitrogen species (RONS), Hemeoxygenase
Labels: MiParea: Respiration, mt-Medicine Pathology: Sepsis Stress:Oxidative stress;RONS Organism: Pig Tissue;cell: Liver, Kidney Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS Pathway: N HRR: Oxygraph-2k