Krumschnabel 1999 Comp Biochem Physiol C Pharmacol Toxicol Endocrinol

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Krumschnabel G, Schwarzbaum PJ, Wieser W (1999) Energetics of trout hepatocytes during A23187-induced disruption of Ca2+ homeostasis. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 124:187-195.

» PMID:10622435

Krumschnabel G, Schwarzbaum PJ, Wieser W (1999) Comp Biochem Physiol C Pharmacol Toxicol Endocrinol

Abstract: The impact of an increase of intracellular Ca2+ i on the energy metabolism of trout hepatocytes was assessed by applying the Ca2+ ionophore A23187 and studying the consequences of the ensuing elevation of Ca2+ i on various metabolic parameters. After application of A23187 no loss of viability occurred for 2 h, but glutathione content decreased by 46%. A concomitant decrease of [ATP] as well as of Na,K-ATPase activity by over 50% could be prevented by incubating the cells in a Ca2+-free medium. Upon addition of the ionophore cellular oxygen consumption more than doubled in a strictly Ca2+-dependent manner, with half of this increase being sensitive to ruthenium red, an inhibitor of the mitochondrial Ca2+ uniporter. This increase in oxygen consumption was transient in nature and at its peak it was similar in magnitude to that induced by 2,4-dinitrophenol. Similarly, oxygen consumption sensitive to the protein synthesis inhibitor cycloheximide was transiently increased by A23187, but returned to control levels within 30 min of incubation. These results suggest that elevation of intracellular Ca2+ leads to an energetic imbalance not related to stimulation of ATP consuming processes, but mainly due to impairment of mitochondrial function, possibly by the decoupling of oxidative phosphorylation and by inducing dissipative Ca2+ cycling. Keywords: Oncorhynchus mykiss, A23187, Ca2, toxicity, ATP, glutathione, protein synthesis, K+ homeostasis

O2k-Network Lab: AT Innsbruck Oroboros

Labels: MiParea: Respiration 

Organism: Fishes  Tissue;cell: Liver  Preparation: Intact cells 

Coupling state: ROUTINE 

HRR: Oxygraph-2k