Krumschnabel 2001 Gen Comp Endocrinol

From Bioblast
Jump to navigation Jump to search
Publications in the MiPMap
Krumschnabel G, Manzl C, Schwarzbaum PJ (2001) Metabolic responses to epinephrine stimulation in goldfish hepatocytes: evidence for the presence of alpha-adrenoceptors. Gen Comp Endocrinol 121:205-213.

» PMID:11178886

Krumschnabel G, Manzl C, Schwarzbaum PJ (2001) Gen Comp Endocrinol

Abstract: The effect of epinephrine on various aspects of cellular metabolism was studied in hepatocytes from the goldfish Carassius auratus. Epinephrine increased cytosolic free calcium ([Ca2+](i)) from a baseline value of 108 +/- 22 nM to a peak value of 577 +/- 127 nM in suspensions of hepatocytes. Responses of single cells ranged from a single spike (66% of hepatocytes) to variable oscillatory patterns (34%). The increase in [Ca(2+)](i) was independent of the presence of extracellular Ca2+ and was prevented by the alpha-adrenergic antagonist phentolamine. Cellular glucose release induced by epinephrine (1.7- to 3.2-fold) was significantly reduced in Ca2+-depleted cells and in the presence of phentolamine, providing evidence for the co-occurrence of alpha-adrenoceptors and a Ca2+-independent, presumably beta-adrenergic, system in these cells. Furthermore, epinephrine stimulated oxygen consumption in a Ca2+-dependent manner, which was not due to stimulated Na(+) pump activity. An increased rate of acid secretion of 50%, evoked by epinephrine, appears to be mediated by enhanced Na(+)/H(+) exchange but did not result in intracellular alkalization

Keywords: epinephrine, goldfish, cytosolic calcium concentration, alpha-adrenoceptor, glucose release

O2k-Network Lab: AT Innsbruck Oroboros


Labels:


Organism: Fishes  Tissue;cell: Liver  Preparation: Intact cells 


Coupling state: ROUTINE 

HRR: Oxygraph-2k