Krumschnabel 2016a Abstract MitoFit Science Camp 2016
|O2k-Protocols: Mitochondrial preparations for HRR.|
Mitochondrial (mt) preparations typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), each of which has its pros and cons. Here, different mt preparations from two mammalian experimental model systems, mouse and beef heart, are evaluated and compared regarding their suitability in HRR. It is shown that different mt preparations may result in almost identical (mouse) or at least comparable (beef) mitochondrial coupling control and respiratory capacities. Further, we show that homogenates produced with the PBI-Shredder provide high quality mt preparations, which may be quickly prepared with very little sample required.
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP
Organism: Mouse Tissue;cell: Heart Preparation: Permeabilized tissue, Homogenate, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k Event: D1 MitoFit Science Camp 2016
1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria.