Krumschnabel 2016a Abstract MitoFit Science Camp 2016

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O2k-Protocols: Mitochondrial preparations for HRR.

Link: MiPNet21.13

Doerrier C, Eigentler A, Krautschneider M, Schoepf B, Fontana-Ayoub M, Krumschnabel G, Gnaiger E (2016)

Event: MitoFit Science Camp 2016 Kuehtai AT

Mitochondrial (mt) preparations typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), each of which has its pros and cons. Here, different mt preparations from two mammalian experimental model systems, mouse and beef heart, are evaluated and compared regarding their suitability in HRR. It is shown that different mt preparations may result in almost identical (mouse) or at least comparable (beef) mitochondrial coupling control and respiratory capacities. Further, we show that homogenates produced with the PBI-Shredder provide high quality mt preparations, which may be quickly prepared with very little sample required.


O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck Gnaiger E


Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP 


Organism: Mouse  Tissue;cell: Heart  Preparation: Permeabilized tissue, Homogenate, Isolated mitochondria 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k  Event: D1  MitoFit Science Camp 2016 

Affiliations

1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria.