Kucera 2015 Oxid Med Cell Longev

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Publications in the MiPMap
Kucera O, Mezera V, Moravcova A, Endlicher R, Lotkova H, Drahota Z, Cervinkova Z (2015) In vitro toxicity of epigallocatechin gallate in rat liver mitochondria and hepatocytes. Oxid Med Cell Longev 2015:476180.

» PMID: 25918582 Open Access

Kucera O, Mezera V, Moravcova A, Endlicher R, Lotkova H, Drahota Z, Cervinkova Z (2015) Oxid Med Cell Longev

Abstract: Epigallocatechin-3-gallate (EGCG) is the main compound of green tea with well-described antioxidant, anti-inflammatory, and tumor-suppressing properties. However, EGCG at high doses was reported to cause liver injury. In this study, we evaluated the effect of EGCG on primary culture of rat hepatocytes and on rat liver mitochondria in permeabilized hepatocytes. The 24-hour incubation with EGCG in concentrations of 10 μmol/L and higher led to signs of cellular injury and to a decrease in hepatocyte functions. The effect of EGCG on the formation of reactive oxygen species (ROS) was biphasic. While low doses of EGCG decreased ROS production, the highest tested dose induced a significant increase in ROS formation. Furthermore, we observed a decline in mitochondrial membrane potential in cells exposed to EGCG when compared to control cells. In permeabilized hepatocytes, EGCG caused damage of the outer mitochondrial membrane and an uncoupling of oxidative phosphorylation. EGCG in concentrations lower than 10 μmol/L was recognized as safe for hepatocytes in vitro.


O2k-Network Lab: CZ Hradec Kralove Cervinkova Z


Labels: MiParea: Respiration 

Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Liver  Preparation: Intact cells, Isolated mitochondria  Enzyme: Complex I, Complex II;succinate dehydrogenase  Regulation: mt-Membrane potential  Coupling state: LEAK, OXPHOS  Pathway: N, S  HRR: Oxygraph-2k 

2016-12