Kunz 1993 FEBS Lett

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Kunz WS, Kuznetsov AV, Gellerich FN (1993) Mitochondrial oxidative phosphorylation in saponin-skinned human muscle fibers is stimulated by caffeine. FEBS Lett 323:188-90.

» PMID: 8495738 Open Access

Kunz WS, Kuznetsov AV, Gellerich FN (1993) FEBS Lett

Abstract: The addition of 15 mM caffeine to saponin-skinned human muscle fibers from M. vastus lateralis caused in the presence of 2 mM ATP an approx. 2-fold stimulation of respiration with glutamate + malate. This effect can be abolished by either the addition of the Ca2+ chelator EGTA, the inhibitor of Ca2+ transport Ruthenium red and the inhibitor of the myosin ATPase vanadate. The caffeine concentration dependency of respiration of fibers coincided with the caffeine-caused stimulation of myosin ATPase activity. The activation of oxidative phosphorylation in saponin-skinned human muscle fibers by caffeine can be explained by a stimulation of myosin ATPase caused by Ca2+ release from sarcoplasmic reticulum.

Keywords: Saponin-skinned muscle fiber, Human skeletal muscle, Oxidative phosphorylation, Caffeine

O2k-Network Lab: DE Magdeburg Gellerich FN


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Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: OXPHOS 

HRR: Oxygraph-2k