Kuznetsov 2021 Biochim Biophys Acta Bioenerg
| Kuznetsov AV, Javadov S, Margreiter R, Grimm M, Hagenbuchner J, Ausserlechner MJ (2021) Structural and functional remodeling of mitochondria as an adaptive response to energy deprivation. Biochim Biophys Acta Bioenerg 1862:148393. https://doi.org/10.1016/j.bbabio.2021.148393 |
Kuznetsov AV, Javadov S, Margreiter R, Grimm Michael, Hagenbuchner J, Ausserlechner MJ (2021) Biochim Biophys Acta Bioenerg
Abstract: Cancer cells bioenergetics is more dependent on glycolysis than mitochondrial oxidative phosphorylation, a phenomenon known as the Warburg Effect. It has been proposed that inhibition of glycolysis may selectively affect cancer cells. However, the effects of glycolysis inhibition on mitochondrial function and structure in cancer cells are not completely understood. Here, we investigated the comparative effects of 2-deoxy-d-glucose (2-DG, a glucose analogue, which suppresses cellular glycolysis) on cellular bioenergetics in human colon cancer DLD-1 cells, smooth muscle cells, human umbilical vein endothelial cells and HL-1 cardiomyocytes. In all cells, 2-DG treatment resulted in significant ATP depletion, however, the cell viability remained unchanged. Also, we did not observe the synergistic effects of 2-DG with anticancer drugs doxorubicin and 5-fluorouracil. Instead, after 2-DG treatment and ATP depletion, mitochondrial respiration and membrane potential were significantly enhanced and mitochondrial morphology changed in the direction of more network organization. Analysis of protein expression demonstrated that 2-DG treatment induced an activation of AMPK (elevated pAMPK/AMPK ratio), increased mitochondrial fusion (mitofusins 1 and 2) and decreased fission (Drp1) proteins. In conclusion, this study suggests a strong link between respiratory function and structural organization of mitochondria in the cell. We propose that the functionality of the mitochondrial network is enhanced compared to disconnected mitochondria.
• Bioblast editor: Gnaiger E
Labels: MiParea: Respiration, mt-Structure;fission;fusion
Pathology: Cancer
Tissue;cell: Other cell lines, HUVEC
Preparation: Intact cells
Regulation: Aerobic glycolysis Coupling state: ET, ROUTINE
HRR: Oxygraph-2k
Warburg effect
