Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Lantier 2020 Diabetes

From Bioblast
Publications in the MiPMap
Lantier Louise, Williams Ashley S, Williams Ian M, Guerin Amanda, Bracy Deanna P, Goelzer Mickael, Foretz Marc, Viollet Benoit, Hughey Curtis C, Wasserman David H (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes 69:1636-49.

» PMID: 32439824

Lantier Louise, Williams Ashley S, Williams Ian M, Guerin Amanda, Bracy Deanna P, Goelzer Mickael, Foretz Marc, Viollet Benoit, Hughey Curtis C, Wasserman David H (2020) Diabetes

Abstract: Insulin resistance due to overnutrition places a burden on energy-producing pathways in skeletal muscle (SkM). Nevertheless, energy state is not compromised. The hypothesis that the energy sensor AMP-activated protein kinase (AMPK) is necessary to offset the metabolic burden of overnutrition was tested using chow-fed and high fat (HF)-fed SkM-specific AMPKα1α2 knockout (mdKO) mice and AMPKα1α2lox/lox littermates (WT). Lean mdKO and WT mice were phenotypically similar. HF-fed mice were equally obese and maintained lean mass regardless of genotype. Results did not support the hypothesis that AMPK is protective during overnutrition. Paradoxically, mdKO mice were more insulin sensitive. Insulin-stimulated SkM glucose uptake was ∼two-fold greater in mdKO mice in vivo. Furthermore, insulin signaling, SkM GLUT4 translocation, hexokinase activity, and glycolysis were increased. AMPK and insulin signaling intersect at mTOR, a critical node for cell proliferation and survival. Basal mTOR activation was reduced by 50% in HF-fed mdKO mice, but was normalized by insulin-stimulation. Mitochondrial function was impaired in mdKO mice, but energy charge was preserved by AMP deamination. Results show a surprising reciprocity between SkM AMPK signaling and insulin action that manifests with diet-induced obesity, as insulin action is preserved to protect fundamental energetic processes in the muscle.

© 2020 by the American Diabetes Association. Keywords: Obesity, Insulin resistance, Muscle glucose uptake, Energy deficit Bioblast editor: Plangger M O2k-Network Lab: US TN Nashville Wasserman DH, US MN Minneapolis Hughey C


Labels: MiParea: Respiration, Genetic knockout;overexpression  Pathology: Diabetes, Obesity 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: OXPHOS  Pathway:HRR: Oxygraph-2k 

2020-06