Lau 2017 Dissertation
|Lau G (2017) Adaptive variation of mitochondrial function in response to oxygen variability in intertidal sculpins (Cottidae, Actinopterygii). Dissertation p148.|
Abstract: Animals are highly dependent on oxygen (O2). The use of O2, however, comes with both advantages and disadvantages. On one hand, O2 is key to the process that produces chemical energy within mitochondria inside the cell, on the other hand the use of O2 generates reactive oxygen species (ROS), which are harmful byproducts. In this thesis, I investigated these two aspects of O2 use at mitochondria from a group of sculpin fishes that are distributed along the marine intertidal zone and are naturally exposed to daily fluctuations of O2. I found that more hypoxia tolerant sculpins improved O2 binding at the level of mitochondria. However, this increased O2 binding was not associated with increased aerobic energy production, and counterintuitively, there was higher ROS generation in more hypoxia tolerant sculpins. It is possible that higher ROS generation in hypoxia tolerant sculpins is part of the strategy in surviving the O2 variable intertidal.
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Hypoxia Organism: Fishes Tissue;cell: Nervous system Preparation: Isolated mitochondria
Regulation: Oxygen kinetics Coupling state: LEAK, OXPHOS, ET Pathway: N, S, CIV, NS, ROX HRR: Oxygraph-2k, O2k-Fluorometer, TPP
2018-01, Amplex UltraRed