Ledur 2020 Sci Rep
|Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218.|
Ledur Pitia Flores, Karmirian Karina, Pedrosa Carolina de Silva Gouveia, Souza Leticia Rocha Quintino, Assis-de-Lemos Gabriela, Martins Thiago Martino, Ferreira Jessica de Cassia Cavalheiro GomesProperty "Was written by" (as page type) with input value "Ferreira Jessica </br>de Cassia Cavalheiro Gomes" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process., de Azevedo Reis Gabriel Ferreira, Silva Eduardo Santos, Silva Débora, Salerno Jose Alexandre, Ornelas Isis Moraes, Devalle Sylvie, Madeiro da Costa Rodrigo Furtado, Goto-Silva Livia, Higa Luiza Mendonca, Melo Adriana, Tanuri Amilcar, Chimelli Leila, Murata Marcos Massao, Garcez Patricia Pestana, Filippi-Chiela Eduardo Cremonese, Galina Antonio, Borges Helena Lobo, Rehen Stevens Kastrup (2020) Sci Rep
Abstract: Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research has revealed that astrocytes are targets of ZIKV. However, the consequences of ZIKV infection, especially to this cell type, remain largely unknown, particularly considering integrative studies aiming to understand the crosstalk among key cellular mechanisms and fates involved in the neurotoxicity of the virus. Here, the consequences of ZIKV infection in iPSC-derived astrocytes are presented. Our results show ROS imbalance, mitochondrial defects and DNA breakage, which have been previously linked to neurological disorders. We have also detected glial reactivity, also present in mice and in post-mortem brains from infected neonates from the Northeast of Brazil. Given the role of glia in the developing brain, these findings may help to explain the observed effects in congenital Zika syndrome related to neuronal loss and motor deficit.
Labels: MiParea: Respiration, mt-Medicine Pathology: Infectious
Organism: Human Tissue;cell: Nervous system Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k
Labels, 2020-02, Virus