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Linearity
Description
Linearity is the ability of the method to produce test results that are proportional, either directly or by a well-defined mathematical transformation, to the concentration of the analyte in samples within a given range. This property is inherent in the Beer-Lambert law for absorbance alone, but deviations occur in scattering media. It is also a property of fluorescence, but a fluorophore may not exhibit linearity, particularly over a large range of concentrations.
Reference: Paul WL (1991) USP perspectives on analytical methods evaluation. Pharm. Technol. 15: 130-141. Elwell CE (1995) A practical users guide to near infrared spectroscopy. Hamamatsu Photonics, 155pp.
MitoPedia concepts:
MitoFit Quality Control System
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry
Contributed by Harrison DK, 2011-11-18