Long 2019 Methods Mol Biol
|Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46.|
Abstract: In this chapter, we describe detailed protocols for measuring high-resolution respirometry on mitochondria extracted from adult whole mouse heart using the Oroboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response to various respiration inhibitions. The method described in this chapter could discern the different respiration rate on mitochondria extracted from two spatially distinct mitochondrial subpopulations, subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria (IFM). These approaches can easily be translated to other cells and tissues.
• Keywords: Intermyofibrillar mitochondria (IFM), Mitochondria extraction, Mitochondrial oxygen consumption, Mouse heart, Oxygraph, Subsarcolemmal mitochondria (SSM) • Bioblast editor: Plangger M • O2k-Network Lab: CN Wuhan Huang K
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse Tissue;cell: Heart Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, CIV, NS, ROX HRR: Oxygraph-2k, O2k-Protocol