Lozano 2019 Oxid Med Cell Longev

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Lozano O, Lázaro-Alfaro A, Silva-Platas C, Oropeza-Almazán Y, Torres-Quintanilla A, Bernal-Ramírez J, Alves-Figueiredo H, García-Rivas G (2019) Nanoencapsulated quercetin improves cardioprotection during hypoxia-reoxygenation injury through preservation of mitochondrial function. Oxid Med Cell Longev 2019:7683051.

» PMID: 31341535 Open Access

Lozano O, Lazaro-Alfaro A, Silva-Platas C, Oropeza-Almazan Y, Torres-Quintanilla A, Bernal-Ramirez J, Alves-Figueiredo H, Garcia-Rivas G (2019) Oxid Med Cell Longev

Abstract: The effective delivery of antioxidants to the cells is hindered by their high metabolization rate. In this work, quercetin was encapsulated in poly(lactic-co-glycolic) acid (PLGA) nanoparticles. They were characterized in terms of its physicochemical properties (particle size distribution, ζ-potential, encapsulation efficiency, quercetin release and biological interactions with cardiac cells regarding nanoparticle association, and internalization and protective capability against relevant challenges). A better delivery of quercetin was achieved when encapsulated versus free. When the cells were challenged with antimycin A, it resulted in lower mitochondrial O2- (4.65- vs. 5.69- fold) and H2O2 rate production (1.15- vs. 1.73- fold). Similarly, under hypoxia-reoxygenation injury, a better maintenance of cell viability was found (77 vs. 65%), as well as a reduction of thiol groups (~70 vs. 40%). Therefore, the delivery of encapsulated quercetin resulted in the preservation of mitochondrial function and ATP synthesis due to its improved oxidative stress suppression. The results point to the potential of this strategy for the treatment of oxidative stress-based cardiac diseases.

Bioblast editor: Plangger M

Labels: MiParea: Respiration, Pharmacology;toxicology 

Stress:Oxidative stress;RONS, Hypoxia  Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells 

Coupling state: ET  Pathway:HRR: Oxygraph-2k 

Labels, 2019-08