Malonate

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Malonate

Description

Malonate (malonic acid) is a competitive inhibitor of succinate dehydrogenase (Complex II). Malonate is a substrate of malonyl-CoA synthase.

Abbreviation: Mna

Reference: MiPNet09.12


Application in HRR

Mna: Malonate (Malonic acid or Propanedioic acid; CH2(COOH)2), Sigma-Aldrich: M1296, store at RT, CAS: 141-82-2, M = 104.06 g·mol-1
Hazard statements: H318; causes serious eye damage
Recommendation: Prepare fresh every day. For info on stability see PubChem.


Preparation of 2 M stock solution (dissolved in H20) for 2-mL O2k-chamber:
  1. Weigh 20.8 mg malonic acid into a Eppendorf tube of appropriate volume (for final volume of 100 µL).
  2. Dissolve in 85 µL 5 M KOH and check pH.
  3. Titrate small volumes (2 µL) of 5 M KOH until you reach a pH of 6.0 (pH of 7.0 would be ideal, however the pH jumps from pH 6.0 to pH 10.0 with only very small excess KOH).
  4. Add H2O to a final volume of 100 µL.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL syringe.
  • Final concentration: 5 mM.


Preparation of 0.5 M stock solution (dissolved in H20) for 0.5-mL O2k-chamber:
  1. Take 50 µL of the 2 M stock solution.
  2. Add 150 µL H2O.
» O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL syringe.
  • Final concentration: 5 mM.


Malonic acid and malonyl-CoA synthase

Communicated by Garcia-Souza Luiz F 2016-11-11
Activity of mt-malonyl-CoA synthase (Witkowski et al 2011) in a mt-preparation may cause stimulation of respiration after titration of malonate (unpublished observation).
Reference
  • Witkowski A, Thweatt J, Smith S (2011) Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis. J Biol Chem 286:33729-36. - »Bioblast link«

MitoPedia topics: Inhibitor