Mandic 2022 J Comp Physiol B

From Bioblast
Publications in the MiPMap
Mandic M, Frazier AJ, Naslund AW, Todgham AE (2022) A comparative and ontogenetic examination of mitochondrial function in antarctic notothenioid species.

Β» J Comp Physiol B 192:737-50. PMID: 36104549 Open Access

Mandic Milica, Frazier Amanda J, Naslund Adnrews W, Todgham Anne E (2022) J Comp Physiol B

Abstract: Notothenioidei fishes have evolved under stable cold temperatures; however, ocean conditions are changing globally, with polar regions poised to experience the greatest changes in environmental factors, such as warming. These stressors have the potential to dramatically affect energetic demands, and the persistence of the notothenioids will be dependent on metabolic capacity, or the ability to match energy supply with energy demand, to restore homeostasis in the face of changing climate conditions. In this study we examined aerobic metabolic capacity in three species, Trematomus bernacchii, T. pennellii and T. newnesi, and between two life stages, juvenile and adult, by assessing mitochondrial function of permeabilized cardiac fibers. Respiratory capacity differed among the adult notothenioids in this study, with greater oxidative phosphorylation (OXPHOS) respiration in the pelagic T. newnesi than the benthic T. bernacchii and T. pennellii. The variation in mitochondrial respiratory capacity was likely driven by differences in the mitochondrial content, as measured by citrate synthase activity, which was the highest in T. newnesi. In addition to high OXPHOS, T. newnesi exhibited lower LEAK respiration, resulting in greater mitochondrial efficiency than either T. bernacchii or T. pennellii. Life stage largely had an effect on mitochondrial efficiency and excess complex IV capacity, but there were little differences in OXPHOS respiration and electron transfer capacity, pointing to a lack of significant differences in the metabolic capacity between juveniles and adults. Overall, these results demonstrate species-specific differences in cardiac metabolic capacity, which may influence the acclimation potential of notothenioid fishes to changing environmental conditions. β€’ Keywords: Metabolic capacity, Mitochondrial function, Notothenioids, Ontogeny, Oxidative phosphorylation, Permeabilized fibers β€’ Bioblast editor: Plangger M

Labels: MiParea: Respiration, Comparative MiP;environmental MiP 

Organism: Fishes  Tissue;cell: Heart  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k 


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