Manzl 2004 Comp Biochem Physiol C Toxicol Pharmacol

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Manzl C, Krumschnabel G, Schwarzbaum PJ, Dallinger R (2004) Acute toxicity of cadmium and copper in hepatopancreas cells from the Roman snail (Helix pomatia). Comp Biochem Physiol C Toxicol Pharmacol 138:45-52.

» PMID:15313445

Manzl C, Krumschnabel G, Schwarzbaum PJ, Dallinger R (2004) Comp Biochem Physiol C Toxicol Pharmacol

Abstract: The toxic effects of cadmium (Cd) and copper (Cu) on cellular metabolism and cell morphology were investigated in isolated hepatopancreas cells from the Roman snail (Helix pomatia). Cell viability was unaffected during 1 h of incubation with 100 microM Cd, but was significantly reduced from 93% in controls to 87% and 85% with 100 microM Cu and 500 microM Cd, respectively. The adverse effect of 500 microM Cd on cell viability was not observed in cells isolated from Cd pretreated snails. Oxygen consumption remained constant in the presence of 100 microM Cu but was inhibited by 38% after 1 h of exposure to 500 microM Cd. Hepatopancreas cells showed enhanced formation of reactive oxygen species when exposed to 100 microM Cu, but not in the presence of Cd. Morphologically, an increase in cell volume of Cd-exposed cells was noted, while cell membrane bleb formation was induced by both metals. The latter may have been induced by metal effects on the actin filamentous network of the cells which showed distinct actin-staining within the blebs at the cell surface. Overall, our data indicate that both Cd and Cu are acutely toxic for hepatopancreas cells form the Roman snail with Cu being more toxic than Cd.

Keywords: actin, cadmium, copper, Helix pomatia, hepatopancreas cells, reactive oxygen species, Roman snail

O2k-Network Lab: AT Innsbruck Oroboros


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Organism: Molluscs 



Coupling state: ROUTINE 

HRR: Oxygraph-2k 

Toxicology