Menegollo 2019 Methods Mol Biol

From Bioblast
Jump to navigation Jump to search
Publications in the MiPMap
Menegollo M, Tessari I, Bubacco L, Szabadkai G (2019) Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells. Methods Mol Biol 1925:223-32.

» PMID:30674030

Menegollo Michela, Tessari Isabella, Bubacco Luigi, Szabadkai Gyorgy (2019) Methods Mol Biol

Abstract: Cytoplasmic and mitochondrial Ca2+ signals couple cellular ATP production to activity-related energy demand. In order to accurately determine the bioenergetic effect of Ca2+ signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.


Bioblast editor: Cardoso Luiza HD


Cited by

  • Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001


Labels:






MitoFit 2021 MgG