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MiPNet04.05 Titration-Injection

From Bioblast
Publications in the MiPMap
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.

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Oroboros (1999-01) Mitochondr Physiol Network

Abstract: Gnaiger E, Rieger G (1999) From step titration to ramp injection: Uncoupling by FCCP with TIP. Mitochondr Physiol Network 04.05.

» Product: TIP2k, Oroboros O2k, O2k-Catalogue

Fully supported by the O2k-Core and control of the TIP2k by the software DatLab: The TIP2k can be programmed for multiple titrations and continuous injections. As an alternative to traditional step titration, the TIP offers the new option of ramp injection, providing maximum resolution of the concentration dependence of oxygen flux. This is illustrated by the recording of cellular respiratory flux as a function of a continuous increase of uncoupler (FCCP) concentration.

Titration: Programmable, automatic titration regimes, with titration volumes of 0.05 to 250 µl, variable titration intervals and duration of titration pulses.

Injection: Steady-state injection: Operation at quasi steady-states by continuous injection of substrates at limiting rates of consumption, providing new flexibility in experimental design by combining the technical advantages of closed and open systems. Programmable injection flows: 0.01 to 25 µl.s-1. Ramp injection (MiPNet04.05, see above): Ramp increase of effector concentrations by "continuous titration". DatLab software for feedback control by the the TIP2k: for steady-state respirometry at selected oxygen levels and pH-stat applications.

O2k-Network Lab: AT_Innsbruck_Oroboros

Labels: MiParea: Respiration, Instruments;methods 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC  Preparation: Intact cells 

Regulation: Uncoupler 

HRR: Oxygraph-2k, TIP2k, O2k-Protocol