MiPNet09.12 Manual titrations of SUIT chemicals

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MiPNet09.12 Manual titrations of SUIT chemicals

Publications in the MiPMap
O2k-Protocols
Manual titrations of SUIT chemicals with 2.0 mL and 0.5 mL chambers of the Oroboros Bioenergetics Analyzer.

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Oroboros (2024-11-25) Mitochondr Physiol Network

Abstract: Oroboros (2024) Manual titrations of SUIT chemicals with 2.0 mL and 0.5 mL chambers of the Oroboros Bioenergetics Analyzer. Mitochondr Physiol Network 09.12(21):1-4.

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Note

Oligomycin: titrations should be performed by 1-2 Β΅L steps (i.e. 5-10 nM experimental concentration of each step). Careful titration of oligomycin is necessary to determine the optimal inhibitory concentration (from 10 to 40 nM) as elaborated in Vernerova et al 2021 and Zdrazilova et al 2022 and to prevent inhibition of ET respiration as detailed in Ruas et al (2016) and Doerrier et al 2018.


Labels: MiParea: Instruments;methods 




Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid 


HRR: O2k-Protocol 

O2k-chemicals and media, ADP 


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