MiPNet12.10 TIP2k-manual

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MiPNet12.10 TIP2k-manual


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O2k-Manual
Titration-Injection microPump TIP2k manual.

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Oroboros (2021-07-08) Mitochondr Physiol Network

Abstract: Gradl L, Gradl P, Gnaiger E (2021) Titration-Injection microPump TIP2k manual. Mitochondr Physiol Network 12.10(09):1-20.

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O2k-Network Lab: AT_Innsbruck_Oroboros


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Default sorting: chronological. Empty fields appear first in ascending order. 
 Was published in yearReferenceOrganismTissue;cell
MiPNet18.10 O2k-Specifications2024-03-26
Oroboros
O2k-specifications for respirometry and comprehensive OXPHOS analysis.
HumanFibroblast
Donnelly 2023 MitoFit2023Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-00022024-11-17 published in Redox Biol.Human
Mouse
Skeletal muscle
Heart
Nervous system
Other cell lines
Baglivo 2022 BEC2022Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioenerg Commun 2022.8. https://doi.org/10.26124/bec:2022-0008HEK
MiPNet12.10 TIP2k-manual2021-07-08
O2k-Manual
Titration-Injection microPump TIP2k manual.
Malagrino 2019 Oxid Med Cell Longev2019Malagrinò F, Zuhra K, Mascolo L, Mastronicola D, Vicente JB, Forte E, Giuffrè A (2019) Hydrogen sulfide oxidation: adaptive changes in mitochondria of SW480 colorectal cancer cells upon exposure to hypoxia. Oxid Med Cell Longev 2019:8102936.HumanEndothelial;epithelial;mesothelial cell
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3Human
Mouse
Rat
Saccharomyces cerevisiae
Heart
Skeletal muscle
Endothelial;epithelial;mesothelial cell
Blood cells
HEK
Platelet
Fisar 2017 Indian J Biochem Biophys2017Fišar Z, Hroudová J, Namrata S, Macečková D, Kopřivová A (2017) Protocols for high-resolution respirometry experiments to test the activity of electron transfer system of pig brain mitochondria. Indian J Biochem Biophys 54:258-72.PigNervous system
MiPNet10.04 CellRespiration2016-08-08
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
MouseBlood cells
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
Human
Schiffer 2016 Am J Physiol Cell Physiol2016Schiffer TA, Peleli M, Sundqvist ML, Ekblom B, Lundberg JO, Weitzberg E, Larsen FJ (2016) Control of human energy expenditure by cytochrome c oxidase subunit IV-2. Am J Physiol Cell Physiol 311:C452-61.HumanSkeletal muscle
HEK
Ivarsson 2016 Physiol Behav2016Ivarsson N, Schiffer TA, Hernández A, Lanner JT, Weitzberg E, Lundberg JO, Westerblad H (2016) Dietary nitrate markedly improves voluntary running in mice. Physiol Behav 168:55-61.MouseSkeletal muscle
MiPNet09.01 O2k-ParadigmShift2015Oxygraph-2k: Paradigm shift and features of high-resolution respirometry. Mitochondr Physiol Network 09.01.
Harrison 2015 J Appl Physiol2015Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. https://doi.org/10.1152/japplphysiol.00146.2015Mouse
Bovines
Heart
Hezel 2015 Redox Biol2015Hezel MP, Liu M, Schiffer TA, Larsen FJ, Checa A, Wheelock CE, Carlström M, Lundberg JO, Weitzberg E (2015) Effects of long-term dietary nitrate supplementation in mice. Redox Biol 5:234-42.MouseSkeletal muscle
Iftikar 2015 Comp Biochem Physiol A Mol Integr Physiol2015Iftikar FI, Morash AJ, Cook DG, Herbert NA, Hickey AJ (2015) Temperature acclimation of mitochondria function from the hearts of a temperate wrasse (Notolabrus celidotus). Comp Biochem Physiol A Mol Integr Physiol 184:46-55.FishesHeart
Abou-Hamdan 2015 Methods Enzymol2015Abou-Hamdan A, Guedouari-Bounihi H, Lenoir V, Andriamihaja M, Blachier F, Bouillaud F (2015) Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol 554:201-28.Human
Pig
Mouse
Rat
Heart
Nervous system
Liver
Kidney
Genital
CHO
Neuroblastoma
Fibroblast
Matallo 2014 Nitric Oxide2014Matallo J, Vogt J, McCook O, Wachter U, Tillmans F, Groeger M, Szabo C, Georgieff M, Radermacher P, Calzia E (2014) Sulfide-inhibition of mitochondrial respiration at very low oxygen concentrations. Nitric Oxide 15:79-84.MouseLung;gill
Macrophage-derived
Schiffer 2014 FASEB J2014Schiffer TA, Ekblom B, Lundberg JO, Weitzberg E, Larsen FJ (2014) Dynamic regulation of metabolic efficiency explains tolerance to acute hypoxia in humans. FASEB J 28:4303-11.HumanSkeletal muscle
Van Bergen 2014 Mitochondrion2014Van Bergen NJ, Blake RE, Crowston JG, Trounce IA (2014) Oxidative phosphorylation measurement in cell lines and tissues. Mitochondrion 15:24-33.Human
Mouse
Nervous system
Blood cells
Reilly 2013 J Exp Biol2013Reilly BD, Hickey AJ, Cramp RL, Franklin CE (2013) Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse. J Exp Biol 217:1087-93.AmphibiansHeart
Skeletal muscle
Smith 2013 Thesis The University of Guelph - Canada2013Smith BK (2013) Investigations into the regulation of skeletal muscle mitochondrial metabolism. Thesis The University of Guelph - Canada 139pp.RatSkeletal muscle
Gnaiger 2012 Mitochondr Physiol Network Bioblast 20122012
Oroboros
Gnaiger E, Meissner B, Laner V, eds (2012) Bioblast 2012: Mitochondrial Competence. Mitochondr Physiol Network 17.12. Oroboros MiPNet Publications, Innsbruck:96 pp. ISBN 978-3-9502399-5-9
Moon 2012 J Biol Chem2012Moon SH, Jenkins CM, Kiebish MA, Sims HF, Mancuso DJ, Gross RW (2012) Genetic ablation of calcium-independent phospholipase A (iPLA) attenuates calcium-induced opening of the mitochondrial permeability transition pore and resultant cytochrome c release. J Biol Chem 287:29837-50.MouseLiver
Lucchinetti 2012 Anesthesiology2012Lucchinetti E, Awad AE, Rahman M, Feng J, Lou PH, Zhang L, Ionescu L, Lemieux H, Thébaud B, Zaugg M (2012) Antiproliferative effects of local anesthetics on mesenchymal stem cells: potential implications for tumor spreading and wound healing. Anesthesiology 116:841-56.Stem cells
Pesta 2012 Methods Mol Biol2012Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3HumanSkeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Lagoutte 2010 Biochim Biophys Acta2010Lagoutte E, Mimoun S, Andriamihaja M, Chaumontet C, Blachier F, Bouillaud F (2010) Oxidation of hydrogen sulfide remains a priority in mammalian cells and causes reverse electron transfer in colonocytes. Biochim Biophys Acta 1797:1500-11.MouseHeart
Nervous system
Liver
Kidney
CHO
Sjoevall 2010 Crit Care2010Sjoevall F, Morota S, Hansson Magnus J, Friberg H, Gnaiger E, Elmer E (2010) Temporal increase of platelet mitochondrial respiration is negatively associated with clinical outcome in patients with sepsis. Crit Care 14:R214.HumanBlood cells
Platelet
Hansson 2010 J Biol Chem2010Hansson Magnus J, Morota S, Teilum M, Mattiasson G, Uchino H, Elmer E (2010) Increased potassium conductance of brain mitochondria induces resistance to permeability transition by enhancing matrix volume. J Biol Chem 285:741-50.RatNervous system
Gnaiger 2008 POS2008Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.MouseBlood cells
Garedew 2005 Eur Shock Soc2005Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The Oroboros O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11.HumanFibroblast
Gnaiger 2001 Respir Physiol2001Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. https://doi.org/10.1016/S0034-5687(01)00307-3Human
Rat
Heart
Liver
Endothelial;epithelial;mesothelial cell
HUVEC
Gnaiger 2000 Proc Natl Acad Sci U S A2000Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. https://doi.org/10.1073/pnas.97.20.11080Rat
Artemia
Crustaceans
Liver
MiPNet04.05 Titration-Injection1999-01
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
HumanEndothelial;epithelial;mesothelial cell
HUVEC
Gnaiger 1998 BTK-HRR1998Gnaiger E, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (Larsson C, Pahlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:89-95.HumanEndothelial;epithelial;mesothelial cell
HUVEC


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