MiPNet15.03 O2k-MultiSensor-ISE

NextGen-O2k

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MiPNet15.03 O2k-MultiSensor-ISE

O2k-MultiSensor system with ion selective electrodes (ISE).

»  »Versions

Oroboros (2018-01-25) Mitochondr Physiol Network

Abstract: Fasching M, Gnaiger E (2018) O2k-MultiSensor system with ion selective electrodes (ISE). Mitochondr Physiol Network 15.03(08):1-26. -

O2k-Manual: The ion selective electrode (ISE) is a modular O2k-MultiSensor extension of the Oroboros O2k. ISE yield a potentiometric (voltage) signal simultaneously with the oxygen signal in both chambers of the O2k. The ISE system consists of separate reference and measuring electrodes selective for hydrophobic cations (TPP⁺, TPMP⁺), Ca²⁺, Mg²⁺, etc. This O2k-Manual describes the handling and application of the ISE system.

» Product: O2k-TPP+ ISE-Module, Oroboros O2k-Catalogue

• Keywords: HRR, O2k-MultiSensor

• O2k-Network Lab: AT_Innsbruck_Oroboros

» Keywords: Force and membrane potential

Fundamental relationships

» Force

» Affinity

» Flux

» Advancement

» Advancement per volume

» Stoichiometric number

mt-Membrane potential and protonmotive force

» Protonmotive force

» Mitochondrial membrane potential

» Chemical potential

» Faraday constant

» Format

» Uncoupler

O2k-Potentiometry

» O2k-Catalogue: O2k-TPP+ ISE-Module

» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE

» TPP - O2k-Procedures: Tetraphenylphosphonium

» Specifications: MiPNet15.08 TPP electrode

» Poster

» Unspecific binding of TPP+

» TPP+ inhibitory effect

O2k-Fluorometry

» O2k-Catalogue: O2k-FluoRespirometer

» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual

» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin

» TMRM - O2k-Procedures: TMRM

O2k-Publications

» O2k-Publications: mt-Membrane potential

» O2k-Publications: Coupling efficiency;uncoupling

This information is part of O2k-Open Support.

» TPP: O2k-technical_support mt-membrane potential

» Calcium: O2k-technical_support

» Other ions beside TPP+ TPMP+, and Ca2+: There are ion selective membranes available, e.g. from Sigma Aldrich, for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.

Labels: MiParea: Instruments;methods 

HRR: Oxygraph-2k, TPP, O2k-Manual 

O2k-MultiSensor, DatLab, DL6, DL6a7