MiPNet18.05 Amplex-Mouse-heart

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O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.

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Oroboros (2019-06-24) Mitochondr Physiol Network

Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6.

The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.

>> Product: Oroboros O2k, O2k-Catalogue
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O2k-Network Lab: AT Innsbruck Oroboros, AT Innsbruck MitoCom


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» Keywords: Hydrogen peroxide



Labels: MiParea: Respiration 


Organism: Mouse  Tissue;cell: Heart  Preparation: Homogenate 

Regulation: Cyt c  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS  HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol 

O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder