Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2019) O2k-FluoRespirometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(03):1-6.
The O2k-Fluorescence LED2-Module is an extension of the Oroboros O2k for combining high-resolution respirometry and fluorometric measurements. We applied the Fluo-Sensor Green for measurement of H2O2 production with Amplex® Ultrared in mouse heart mitochondria. Experiments are selected to demonstrate the importance of applying ET-pathway competent substrate states, which are critically evaluated by respiratory performance and considering experimental oxygen levels when measuring mitochondrial H2O2 production.
- » Keywords: Hydrogen peroxide
Labels: MiParea: Respiration
Organism: Mouse Tissue;cell: Heart Preparation: Homogenate
Regulation: Cyt c Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder