MiPNet19.03 O2k-cleaning and ISS

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MiPNet19.03 O2k-cleaning and ISS

O2k-Chamber cleaning SOP and Integrated Suction System (ISS).

»  » Versions

Oroboros (2020-10-13) Mitochondr Physiol Network

Abstract: Di Marcello M, Gnaiger E, Komlodi T (2020) O2k-Chamber cleaning SOP and Integrated Suction System (ISS). Mitochondr Physiol Network 19.03(06):1-8.

O2k-chamber

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» Current O2k-series: O2k-FluoRespirometer

» Current software version: DatLab 7.4

• Further details: » MitoPedia: O2k-Open Support

» Product: ISS-Integrated Suction System

• O2k-Network Lab: AT_Innsbruck_Oroboros

Additional resources

» Chemicals contamination

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» O2k-chamber

» The experimental chamber in the instrumental O2k-Chamber

» O2k-Chamber sV

» Calibration of O2k-chamber volume

• On chamber volume

» O2k-chamber

» Chamber volume

» MiPNet09.01 O2k-ParadigmShift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental O2k-chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» O2k-cleaning and ISS

» Quality control: O2k-chamber test

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

» Oxygen signal

» O2k signal line

O2k-Procedures

» Air calibration

» Zero calibration

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

» DL-Protocols

» Oxygen sensor test

» Stirrer test

» Open chamber

» Closed chamber

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

Labels: MiParea: Instruments;methods 

HRR: Oxygraph-2k, O2k-Manual 

O2k-SOP