MiPNet19.15 IOC97

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MiPNet19.15 IOC97

Publications in the MiPMap
O2k-Network

Lausanne CH, 2014 Sep. 25-26 Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC97.

» Bioblast pdf

Oroboros (2014-09-25) Mitochondr Physiol Network

Abstract:
O2k-Workshops

97th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry


O2k-Network Lab: CH Lausanne Sandi C, AT Innsbruck Oroboros


Labels:





HRR: Oxygraph-2k 

ORO, IOC, 2014, O2k-Network 


» MitoPedia: Respiratory states OXPHOS ROUTINE ETS LEAK ROX

Lecturer

Erich Gnaiger Gnaiger Erich, CEO, Oroboros Instruments


Programme

Programme MiPNet19.15.pdf

Thursday, September 25

09:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k.

  • Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi).

10:30 Break – Registration of external participants

11:00 – 11:15 Erich Gnaiger:

Welcome to external participants. Oroboros Instruments and perspectives of mitochondrial physiology.

11:15 – 11:30 Introduction of participants: who is who?

11:30 – 12:30 Basic and new features of DatLab 6.

  • Oxygen calibration.
  • Oxygen flux and instrumental tests.

12:30 Lunch

13:30 – 15:30 Experimental design.

  • Cell ergometry: from intact cells to mt-preparations.
  • A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle.

15:30 Break

16:00 – 17:30 Titration-Injection microPump TIP2k:

From automatic instrumental background tests to steady-state control in respiratory experiments.

17:30 – 18:00 Q&A session on HRR and OXPHOS analysis

Design of experimental protocol - day 2.


Friday, September 26

09:00 – 10:30 Experiment

HRR and O2k-Fluorometry – respiration and H2O2 production.

10:30 Break

11:00 – 12:30 Experiment continued.

12:30 Lunch

13:15 – 15:30 DatLab analysis.

15:30 Break

16:00 – 16:30 Trouble shooting.

16:30 – 17:30 Normalization of mt-respiration: flux control states and flux control steps.

17:30 - 18:00 Feedback – conclusions – stay connected as a MiPNet Lab.


Venue

Laboratory of Behavioral Genetics

Brain Mind Institute

Ecole Polytechnique Federale de Lausanne


Auditorium/Meeting room

CH-1015 Lausanne

Contact: Prof. Carmen Sandi, Director. Email: carmen.sandi@epfl.ch


Registration and general information

instruments@oroboros.at


Oroboros Instruments

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20


Participants

Name Institution
Canto_AC (Thursday, Friday) CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL
Eggimann Sandra CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern
Grosse Jocelyn (Thursday) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Hahn Dagmar CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern
Hollis Fiona (Thursday afternoon and Friday) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Huzard Damien (Thursday morning and Friday morning) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Meng Kévin (Thursday afternoon and Friday morning) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Kulkarni Sameer CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL
Papilloud Aurélie (Thursday and Friday) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Ratajczak Joanna (Thursday afternoon and Friday) CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL
Riccio Orbicia (Thursday afternoon and Friday) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Sandi Carmen CH Lausanne Sandi C: Brain Mind Institute, EPFL
Walker Sophie (Thursday) CH Lausanne Sandi C: Brain Mind Institute, EPFL
Zanoletti Olivia (Thursday and Friday) CH Lausanne Sandi C: Brain Mind Institute, EPFL


Recommended reading

Key references

O2k-Publications in the MiPMap
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access


Introduction and O2k-Core Manual

O2k-Manual
»O2k-Core Manual.pdf


HRR and O2k-Fluorometry

O2k-Fluorometer
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Pubmed 24862266


Mitochondrial pathways

O2k-Protocols
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. »Open Access


MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.