MiPNet27.06 Prague BEC tutorial-Living Communications pmF

Prague CZ, 2022 Sep 22. BEC tutorial-Living Communications: pmF — pre EMC2022 Prague.

MiPsociety (2022-09-22) Mitochondr Physiol Network

Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics. Preceding the EMC 2022 49th European Muscle Conference, Prague, Czech Republic.

The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields 40 000 results and 3452 for 2021 (search 2022-09-20), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for an introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks on potential gradients, Gibbs energy, protonmotive flow and force, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets tough, join for a follow-up retreat.

• O2k-Network Lab: AT_Innsbruck_Oroboros, CZ Prague Houstek J

Figure 1.1. Coupling in oxidative phosphorylation is mediated by the protonmotive force pmF.

Last update: 2022-09-20

This MiPevent is dedicated to Dr. Zdenek Drahota — one of the greatest mitochondrial physiologists of the Czech Republic — at his 90^th birthday:

In collaboration with the Mitochondrial Physiology Society - see MiP2017

Venue and local organizers

Laboratory of Bioenergetics

Institute of Physiology CAS

Videnska 1083

142 20 Prague 4

Czech Republic

Zuzana Korandová, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
Tomáš Mráček, RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
Pecina Petr, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
Josef Houstek, Prof, MD, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ

Program

Blocks of 10 + 5 min comments, questions, discussion
Sections of Chapter 8, Blue Book

**Thursday, Sep 22** — Protonmotive force *pmF* = Δ_mF_H⁺ and **protonmotive pressure Δ_mΠ_H⁺**
Time	Section	Topic	a	b	c	d
10:00-10:15	8.	Overview: from Mitchell's four modules to four protonmotive theorems	diffusive pressure gradient	pressure difference and potential difference	diffusive and electric pressure difference	concave to convex flow/force
10:15-10:25	8.1.	1. Vectorial and scalar quantities	amount and charge format	diffusion: gradients and compartments	stoichiometry and advancement
10:30-10:40	8.2.	2. Why is thermodynamics scary?	chemical potential	metabolic force	Gibbs energy - exergy
10:45-10:50	8.2.	3. The elementary unit	SI base units	elementary quantities	count on motive units	beyond the Gas constant: Boltzmann, Avogadro, Faraday
11:00-11:10	8.3.	4. Protonmotive force and motive units	Why Δp?	pmF formats	conversion between formats	measurement of the pmF
11:15-11:45		Coffee/tea - Chat
11:45-11:55	8.4.1.	*5. Protonmotive pressure pmP* linearity**	diffusion gradients and Einstein's diffusion equation	Fick's law	pressure-force confusion	thermodynamics of irreversible processes
12:00-12:10	8.4.2.	6. Compartments: diffusion and osmotic pressure	concave flux/force relation	free activity	infinite forces without explosion
12:15-12:25	8.4.3.	7. Hydrogen ions and counterions	if the force is in ∆pH	electroneutral exchange of counterions	equilibrium H⁺ and counterion distribution	concave flow (pressure)/force relation
12:30-12:45	8.4.4.	8. Matrix volume fraction and flux-pressure linearity	anodic volume fraction	closed to open anodic system	from intensity to capacity	non-ohmic proton leak explained by first principles
12:45-13:00		General discussion with a glass of wine - a taste of Gentle Science

» Programme: BEC tutorial-Living Communications: pmF to pmP

Lecturer and participants

Erich Gnaiger, PhD., Oroboros Instruments - author of
Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis.
Chapter 8. Protonmotive pressure and respiratory control.

Participants

Restricted number of participants: count_max = (29 + 1) x

1. Lukáš Alán, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
2. Zuzana Červinková, Prof, MD, PhD, Lab Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
3. Eva Doleželová, PhD, Institute of Parasitology, Ceske Budejovice, CZ
4. Zdenek Drahota, PhD, Lab of Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
5. Jan Eliáš, MBBCh, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
6. Moustafa Elkalaf, MBBCh, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
7. René Endlicher, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
8. František Galatík, Laboratory of Physiology, Faculty of Science, Charles University, Prague, CZ
9. Olga Horakova, Laboratory of Adipose Tissue Biology, Institute of Physiology, Czech Academy of Sciences, CZ
10. Josef Houstek, Prof, MD, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
11. Petra Janovska, PhD, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
12. Petr Kašík, Faculty of Science, Charles University, Prague, CZ
13. Michal Knězů, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
14. Eliška Koňaříková, Dr. rer. nat., Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
15. Zuzana Korandová, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
16. Barbora Kudrnovská, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
17. Tomáš Mráček, RNDr, Head of Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
18. Petr Pecina, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
19. Alena Pecinova, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
20. Guillermo Puertas Frias, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
21. Maria Jose Saucedo Rodriges, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
22. Ondrej Sobotka, MUDr, PhD, Faculty of Medicine, Charles University, Hradec Kralove, CZ
23. Pavla Staňková, Mgr, PhD, Lab of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, CZ
24. Kateřina Tauchmannová, PhD, Lab Bioenergetics, Institute of Physiology, Czech Academy of Sciences, Prague, CZ
25. Lucie Zdrazilova, MSc, First Faculty of Medicine,Charles University, Prague, CZ
26. Petr Zouhar, Institute of Physiology, Czech Academy of Sciences, Prague, CZ

Registration and general information

Informal, no registration fee - send Email to: instruments@oroboros.at
Limited number of participants
Provide your name and affiliation (if you wish for the website)
Provide a foto (if you wish for the website)

COVID-19

The event will be held in accordance with current COVID regulations. A primary concern must be the safety of our participants and staff, which is why we reserve the right to cancel the event if there are any concerns/restrictions.

Contents

Venue and local organizers

Program

Lecturer and participants

Registration and general information

Recommended reading