MitoPedia: O2k-FluoRespirometry

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MitoPedia: O2k-FluoRespirometry


Template NextGen-O2k.jpg
O2k-Open Support


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



MitoPedia: O2k-FluoRespirometry is a component of O2k-Open Support.
It lists specific fluorometry-related topics.
See also
» MitoPedia: O2k-Respirometry
TermAbbreviationDescription
Cell count and normalization in HRRNceThe cell count Nce is the number of cells, expressed in the abstract unit [x] (1 Mx = 106 x). The elementary entity cell Uce [x] is the real unit, the 'single individual cell'. A cell count is the multitude or number N of cells, Nce = N·Uce (Gnaiger MitoFit Preprints 2020.4). Normalization of respiratory rate by cell count yields oxygen flow IO2 expressed in units [amol·s-1·x-1] (=10-18 mol·s-1·x-1).
Cover-Slip\black
Cover-Slip black.JPG
A Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the O2k.
DatLab
DatLab
DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k-FluoRespirometer.

The newest DatLab version is DatLab 7.4, included in the O2k-FluoRespirometer.


The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP. However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.
Electrolyte\Reference-Electrode
Electrolyte Reference-Electrode.jpg
Electrolyte\Reference-Electrode for Reference-Electrode\2.4 mm
Filter Set AmR
Filter Set AmR.JPG
Filter Set AmR: Set of filters for the determination of H2O2 production with Amplex UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter Set MgG / CaG
Filter Set MgG CaG.JPG
Filter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter Set Saf
Filter Set Saf.JPG
Filter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter-Cap
Filter-Cap.JPG
Filter-Cap: O2k-Fluo LED2-Module (O2k-Series D to G) sensors (Fluorescence-Sensor Green and Fluorescence-Sensor Blue) and O2k-FluoRespirometer (O2k-Series H to I) sensors (Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.
Fluorescence-Control Unit
Fluorescence-Control Unit lettered.jpg
Fluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the LED in the fluorescence sensors. This item is a standard component of the O2k-Fluorescence LED2-Module.
Fluorescence-Sensor Blue
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor Blue: excitation LED 465 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. Filter sets for Magnesium green® / Calcium green® measurements are included.
Fluorescence-Sensor Green
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor Green: excitation LED 525 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered.
Fluorescence-Service Box
Fluorescence-Service Box.JPG
Fluorescence-Service Box: for storage and shipment of theO2k-Fluo LED2-Module.
Forceps for membrane application
Forcep for membrane application.jpg
Forceps for membrane application: for OroboPOS and ISE membrane application; do not use for tissue preparation.
Forceps\stainless Steel\angular Tip\fine
Forcep for tissue preparation angular tip.jpg
Forceps\stainless Steel\angular Tip\fine: for tissue preparation, stainless steel. Two pairs are used particularly for muscle fiber separation.
Forceps\stainless Steel\rounded Tip\sharp
Forcep for tissue preparation rounded tip.jpg
Forceps\stainless Steel\rounded Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for placing the tissue sample onto the microbalance and for handling in combination with Forceps\stainless Steel\straight Tip\sharp.
Forceps\stainless Steel\straight Tip\sharp
Forcep for tissue preparation straight tip.jpg
Forceps\stainless Steel\straight Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for insertion of the sample into the O2k-chamber and for handling in combination with Forceps\stainless Steel\rounded Tip\sharp.
High-resolution respirometryHRR
O2k-FluoRespirometer
High-resolution respirometry, HRR, is the state-of-the-art approach in mitochondria and cell research to measure respiration in various types of mitochondrial preparations and living cells combined with MultiSensor modules. Mitochondrial function and dysfunction have gained an increasing interest over the past years, reflecting growing awareness of the fact that mitochondria play a pivotal role in human health and disease. Combining instrumental accuracy and reliability with versatility of applicable protocols - allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers - mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. The most advanced way to analyze mitochondrial function is by means of high-resolution respirometry with the Oroboros O2k. The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. Substrate-uncoupler-inhibitor titration (SUIT) protocols allow the diagnosis of numerous mitochondrial pathway and coupling defects in a single respirometric assay. Technologically, HRR is based on the Oroboros O2k, combining optimized chamber design, application of oxygen-tight materials, electrochemical sensors, Peltier-temperature control, and specially developed software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed-chamber or open-chamber mode of operation (TIP2k). Standardized calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. HRR can be extended for MultiSensor analysis by using the O2k-FluoRespirometer. Smart Fluo-Sensors are integrated into the O2k to measure simultaneously fluorometric signals using specific fluorophores. Potentiometric modules are available with ion selective electrodes (pH, TPP+). The NextGen-O2k is the all-in-one device including the Q-redox sensor and a PhotoBiology (PB) module.
ISE Package 1 TPP or Ca
ISE Package 1 TPP or Ca.JPG
O2k-TPP+ and Ca2+ ISE\1 Chamber: ISE-Package for 1 TPP+ and Ca2+ electrode.
ISE-Ca2+ Membranes
Ca2+ membranes.jpg
ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes. To be used with the O2k-TPP+ ISE-Module.
ISE-Compressible Tube
ISE-Compressible Tube.JPG
ISE-Compressible Tube for Ion-Selective Electrode TPP+ and Ca2+.
ISE-Filling Syringe
ISE-Filling Syringe.JPG
ISE-Filling Syringe with needle for Ion-Selective Electrode TPP+ and Ca2+.
ISE-Inner Glass Electrode
ISE-Inner Glass Electrode.JPG
ISE-Inner Glass Electrode of ISE, with Ag/AgCl- and Pt-wire
ISE-Membrane Mounting Tool
ISA-Membrane Mounting Tool.JPG
ISE-Membrane Mounting Tool for Ion-Selective Electrode TPP+ and Ca2+. O2k-TPP+ ISE-Module: mounting tool included.
ISE-Membrane Seal
ISE-Membrane Seal.JPG
ISE-Membrane Seal for Ion-Selective Electrode TPP+ and Ca2+.
ISE-Service BoxISE-Service Box: for storage and shipment of the O2k-TPP+ ISE-Module or O2k-pH ISE-Module.
ISE-TPP+ Membranes
ISE-TPP+ Membranes.JPG
ISE-TPP+ Membranes, PVC, 4 mm diameter, box of 5 membranes.
ISS-Filter and Tubing
ISS-Filter and Tubing.JPG
ISS-Filter and Tubing, ISS-Integrated Suction System.
ISS-Integrated Suction System
ISS.jpg
ISS-Integrated Suction System: Suction pump with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-chambers. The ISS is included as a standard component of the O2k-FluoRespirometer. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.
ISS-Lid
ISS-Lid.JPG
ISS-Lid, for ISS-Waste Bottle, component of the ISS-Integrated Suction System.
ISS-Waste Bottle
ISS-Waste Bottle.JPG
ISS-Waste Bottle, 2-liter, component of the ISS-Integrated Suction System.
Ion-Selective Electrode TPP+ and Ca2+
Ion-Selective Electrode TPP+ and Ca2+.JPG
Ion-Selective Electrode TPP+ and Ca2+: ISE with 6 mm outer diameter shaft, for Stopper\white PVDF\angular Shaft\side+6.2+2.6 mm Port. O2k-TPP+ ISE-Module: 2 ISE.
MiR05-Kit
MiR05-Kit.jpg
Mitochondrial Respiration Medium - MiR05-Kit, 1 vial; for a final volume of 250 mL
Microbalance 120 g
Microbalance 120 g.jpg
Microbalance 41 g to 120 g; 0.01 mg / 0.1 mg display; particularly for wet weight determination of permeabilized fibres.
Microbalance-Transport Case
Microbalance-Transport Case.jpg
Microbalance transport case, not suitable for shipping
Microsyringe\10 mm3 51/0.13 mm
Hamilton Syringes for Manual Titration.jpg
Hamilton Microsyringe\10 mm3 51/0.13 mm for manual titrations, 10 mm3 volume; fixed injection needle with rounded tip: 51 mm length, 0.13 mm inner diameter.
Microsyringe\100 mm3 51/0.41 mm
Microsyringe 100 mm3 51 0.41 mm - Kopie.JPG
Hamilton Microsyringe\100 mm3 51/0.41 mm for manual titrations, 100 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter. It is recommended for injections of suspensions of isolated mitochondria.
Microsyringe\200 mm3\TIP2k
Microsyringe200 mm3TIP2k.JPG
Microsyringe\200 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 200 mm3 (µl), fixed injection needle with rounded tip, with spacers.
Microsyringe\25 mm3 51/0.15 mm
Microsyringe 25 mm3 51 0.15 mm.JPG
Hamilton Microsyringe\25 mm3 51/0.15 mm for manual titrations, 25 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.15 mm inner diameter.
Microsyringe\50 mm3 51/0.15 mm
Microsyringe 100 mm3 51 0.41 mm - Kopie.JPG
Hamilton Microsyringe\50 mm3 51/0.15 mm for manual titrations, 50 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.15 mm inner diameter.
Microsyringe\500 mm3\TIP2k
Microsyringe 500 mm3 TIP2k.JPG
Microsyringe\500 mm3\TIP2k: Microinjection syringe for Titration-Injection microPump, 500 mm3 (µl), fixed injection needle with rounded tip, with spacers.
MitoKit-CII
MITOKIT-CII.jpg
Cell permeable prodrugs, composed of MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv, stimulates (Snv) or inhibits (Mnanv) mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres, acting on Complex II of the electron transfer system.
Motic Microscope
Motic Microscope.jpg
Motic Microscope SMZ-171 TP: for preparation of permeabilized fibres; compact and light stereo microscope, Greenough optical system, switching power supply for use worldwide (100-240V); including auxiliary ESD objective 2.0X(38.6mm).
O-ring sV\Viton\9.5x1 mm
34310-02.jpg
O-ring sV\Viton\9.5x1 mm, for PEEK Stopper sV, 2 are mounted on each PEEK Stopper sV, box of 8 as spares.
O-ring\Viton\12.5x1 mm
O-ringViton12.5x1 mm.jpg
O-ring\Viton\12.5x1 mm, for PVDF or PEEK O2k-Stoppers (2-mL O2k-Chamber), box of 8 as spares.
O-ring\Viton\18x2 mm
Viton O-ring 18x2.jpg
O-ring\Viton\18x2 mm, mounted on the O2k-Chamber Holder.
O-ring\Viton\6x1 mm
POS O-ring for sensor head or POS mounting tool.jpg
O-ring\Viton\6x1 mm for OroboPOS-Mounting Tool.
O-ring\Viton\8x1 mm
POS O-ring for sensor head or POS mounting tool.jpg
O-ring\Viton\8x1 mm: for OroboPOS sensor head. Replaces the O-ring\Viton\9x1 mm
O2-Zero Powder
O2-Zero Powder.jpg
O2-Zero Powder, dithionite (Na2S2O4), for zero-oxygen calibration of the OroboPOS.
O2k-Assembly KitThe O2k-Assembly Kit is a component of the O2k-FluoRespirometer, consisting of 2 PVDF Stirrer-Bars, 2 PVDF or PEEK O2k-Stoppers, OroboPOS-Connectors (attached to the O2k-Main Unit) and cables (power supply, USB-connection). Several components of the O2k-Catalogue: O2k-Assembly Kit are included in the O2k-Service Box either for shipment or for storage.
O2k-Barometric Pressure TransducerO2k-Barometric Pressure Transducer, A/D converter and digital output to DatLab for continuous recording of barometric pressure [kPa or mmHg], integrated into the air calibration of the OroboPOS (MiPNet06.03 POS-calibration-SOP). Integral component of the O2k-Main Unit. The warranty on the accuracy of the signal obtained from the O2k-Barometric Pressure Transducer expires within three years.
O2k-Chamber Holder
Chamber holder PVDF Stopper.jpg
O2k-Chamber Holder (blue POM) for PVDF or PEEK stoppers (2-mL O2k-chamber), with O-ring\Viton\18x2 mm and V-ring\30-35-4.5 mm. Two units of this item are standard components mounted on the O2k-Main Unit.
O2k-Chamber sV
33100-01.jpg
O2k-Chamber sV: 12 mm inner diameter, Duran® glass polished, with standard operation volume V of 0.5 mL.
O2k-Dissection Set
O2k-Dissection-Set.jpg
O2k-Dissection Set: for tissue preparation, set of 4 pairs of stainless steel, antimagnetic forceps and a pair of scissors.
O2k-Fluo LED2-Module
Fluorescence-Control Unit lettered.jpg
The O2k-Fluo LED2-Module is a component of the O2k-Fluorometer (O2k-Series D to G). It is an amperometric add-on module to the O2k-Core (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex® UltraRed), ATP production (Magnesium Green™), mt-membrane potential (Safranin, TMRM, Rhodamine 123), Ca2+ (Calcium Green™), and numerous other applications open for O2k-user innovation.
O2k-Fluo Smart-Module
12100-03.jpg
The O2k-Fluo Smart-Module is a component of the O2k-FluoRespirometer (O2k-Series H - I). It is an amperometric add-on module to the O2k-Respirometer, adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex® UltraRed), ATP production (Magnesium Green™), mt-membrane potential (Safranin, TMRM), Ca2+ (Calcium Green™), and numerous other applications open for O2k-user innovation.
O2k-FluoRespirometer
O2k-FluoRespirometer
The Oroboros O2k-FluoRespirometer - the experimental system complete for high-resolution respirometry (HRR), including fluorometry, the TIP2k and the O2k-sV-Module allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG). The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+) or pH.
O2k-Fuse Power Plug\M2.5 A\5x20 mm
Fuses mains.jpg
O2k-Fuse Power Plug\M2.5 A\5x20 mm: This item is a standard component of the O2k-Assembly Kit (O2k-FluoRespirometer), mounted on the socket for the O2k-Main Power Cable, at the rear panel of the O2k-Main Unit.
O2k-Main Power CableO2k-Main Power Cable, for connecting the main unit to the power supply.
O2k-Main Power Cable\120 V\US-CA
O2k-Main Power Cable 120 V US-CA.JPG
O2k-Main Power Cable\120 V\US-CA, USA and Canada (120 V).
O2k-Main Power Cable\230 V\AU-NZ
O2k-Main Power Cable 230 V AU-NZ.JPG
O2k-Main Power Cable\AU-NZ, Australia and New Zealand (230 V).
O2k-Main Power Cable\230 V\Europe
O2k-Main Power Cable 230 V Europe.JPG
O2k-Main Power Cable\230 V\Europe.
O2k-Main UnitThe O2k-Main Unit is a component of the O2k-Core. The O2k-Main Unit consists of functionally defined, integral elements, the (O2k-Main Basic, O2k-Peltier Temperature Control, two O2k-Electromagnetic Stirrer Twin-Control units, two O2k-Amperometric OroboPOS Twin-Channels, O2k-Barometric Pressure Transducer), which cannot be obtained separately.
O2k-Service BoxO2k-Service Box empty, contains components of the OroboPOS-Service Kit and the O2k-Assembly Kit.
O2k-Specifications
O2k-Info.png
O2k versus multiwell respirometer: O2k stands for Oroboros O2k and high-resolution respirometry, meeting powerful quality criteria securing high output and pioneering state-of-the-art comprehensive OXPHOS analysis of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. If you’re using a biased instrument, it doesn’t matter how many measurements you take – you’re aiming at the wrong target (Silver 2012 Penguin Press).
O2k-Titration Set
O2k-Titration Set.JPG
The O2k-Titration Set consists of Hamilton microsyringes (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3; fixed needles with rounded tips), provided in the Syringe Storage Box with Syringe Labels, a set of two Syringe Racks with Syringe Collars, and a set of two Tube Racks. The O2k-Titration Set is included in the O2k-FluoRespirometer and Startup O2k-Respirometer.
O2k-USB Flash Drive
Oroboros-USB-flash-drive.JPG
The O2k-USB Flash Drive is a component of the Oroboros O2k containing: DatLab, O2k-Manual, O2k-Protocols, O2k-Publications, and info on O2k-Workshops.
O2k-Window Frame
O2k-Window Frame.JPG
O2k-Window Frame: blue POM, with thread for fixation on the O2k-Main Unit, to be removed only for rare cleaning purposes and for front fixation of the Fluorescence-Control Unit, using the O2k-Window Tool.
O2k-Window Tool
O2k-Window Tool.jpg
O2k-Window Tool for removing the blue O2k-Window Frame from the O2k-Main Unit, for rare cleaning purposes and for front fixation of the Fluorescence-Control Unit.
O2k-chamber
O2k-Chamber.jpg
O2k-Chamber: Duran® glass polished, with standard operation volumes (V) of 2.0 mL or 0.5 mL (small chamber volume in the O2k-sV-Module, 12 mm inner diameter). The optical properties of Duran® allow application of fluorometric sensors (Duran® optical properties).
O2k-sV-ModuleO2k-sV-Module
11200-01.jpg
The O2k-sV-Module is the O2k small-volume module, comprised of two Duran® glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all the components necessary for a smaller operation volume V of 0.5 mL. The current DatLab version is included in the delivery of this revolutionary module.
OroboPOS
OroboPOS.JPG

The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.

It consists of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.
OroboPOS-Connector
POS connector and cable connection.jpg
OroboPOS-Connector (blue POM), with male connection to OroboPOS sensor head (POS) and with cable and male plug fitting into O2k-Main Unit.
OroboPOS-Connector ServiceThe OroboPOS-Connector Service entails routine maintenance and any necessary repairs of the OroboPOS-Connector in the Oroboros electronics workshop (WGT).
OroboPOS-Electrolyte Powder
POS Electrolyte powder.jpg
OroboPOS-Electrolyte Powder, KCl. The powder is dissolved in 10 ml distilled water to yield a 1 M KCl solution.
OroboPOS-Holder
Pos holder.JPG
The OroboPOS-Holder (POS-Holder), made from blue POM, is screwed into the copper block of the O2k-Main Unit, guiding the OroboPOS-Connector with the OroboPOS sensor head (POS) to the 2-mL O2k-chamber, and keeping the OroboPOS-Connector in a fixed position for sealing the O2k-chamber with the OroboPOS-Seal Tip. In addition, the OroboPOS-Holder fixes the O2k-Chamber in an accurate rotational position by pressing against the angular cut of the glass chamber. Two units of this item are standard components mounted on the O2k-Main Unit.
OroboPOS-Membrane Ring
POS membrane holder ring.jpg
OroboPOS-Membrane Ring, PEEK, holds the membrane against the inner O-ring on the POS housing.
OroboPOS-MembranesFEP-membranes
POS membranes.jpg
OroboPOS-Membranes, FEP 25 µm; 40/Pck.
OroboPOS-Mounting Tool
POS mounting tool for membrane application 02.JPG
OroboPOS-Mounting Tool for application of OroboPOS-Membranes. It consists of two parts, (i) the membrane guide (larger diameter) and (ii) the membrane ring holder with O-ring\Viton\6x1 mm for holding the OroboPOS-Membrane Ring during membrane application. Since the O2k-Series G-0075 membrane ring holder have a 2 mm extended ridge for a better grip.
OroboPOS-Polishing Cloth
POS polishing cloth for cathode cleaning.jpg
OroboPOS-Polishing Cloth for cathode cleaning. Replace the OroboPOS-Polishing Cloth at intervals. A two-year interval may be considered in cases of intensive use.
OroboPOS-Polishing Powder
POS polishing powder for cathode cleaning.jpg
OroboPOS-Polishing Powder for cathode cleaning.
OroboPOS-Seal Tip
POS seal tip.jpg
OroboPOS-Seal Tip, black butyl rubber gasket with 3 mm pore. 4/Pck, for sealing the OroboPOS (sensor head) against the O2k-Chamber. Push the wetted OroboPOS-Seal Tip over the POS-sensor head, with the pore positioned centrally, not covering any peripheral area of the gold cathode. Do not stretch the gasket across the POS-sensor head.
OroboPOS-Service Kit
ServiceBox G 2.jpg
OroboPOS-Service_Kit, with O2k-Service Box including all oxygen sensor service accessories for membrane mounting and service of the OroboPOS.
Oroboros O2k
O2k-FluoRespirometer
O2k-Core to FluoRespirometer

Oroboros O2k - the modular system for high-resolution respirometry (HRR) for mitochondria and cell research. The O2k-Core (O2k-Series D - G with the O2k-Fluo LED2-Module) and the O2k-FluoRespirometer (O2k-Series H - I) are the basis for combined measurement of respiration and fluorometric signals.

Small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices.

The modular O2k-concept is supported by DatLab 7, with high flexibility for extension by add-on O2k-Modules or economical first steps with the Startup O2k-Respirometer. The high-resolution O2k technology is world-wide tested and trusted with emphasis on quality and scientific output of research in mitochondrial physiology and pathology.
PBI-Shredder O2k-Set
PBI-Shredder HRR-Set.JPG
PBI-Shredder O2k-Set: Auxiliary O2k-Tool for tissue homogenate preparation
PBI-Shredder SG3
PBI-Shredder SG3.jpg
PBI-Shredder SG3 for tissue homogenate preparation, heavy duty high torque SG3 driver with convertible handle, SG3 base with 3 position force setting lever (FSL), battery charger and two lithium ion batteries. The PBI-Shredder SG3 is included in the PBI-Shredder O2k-Set. Select 230 V or 120 V. Oroboros Instruments: world-wide distributor.
PH-Glass electrode
PH-Glass Electrode.JPG
pH-Glass electrode with BNC plug.
Packing\O2k-Box 1
Packing O2k-Box1.JPG
Packing\O2k-Box 1: for shipment of the O2k-Main Unit, with polystyrene inlets. Keep the original packing material safely stored, for any future shipping purposes of the O2k-Main Unit.
Packing\O2k-Box 1+2Packing\O2k-Box 1+2 for shipping the O2k-Core. O2k-WorldWide delivery, insurance and handling are included in the O2k-Core.
Packing\O2k-Box 2
Packing O2k-Box2.JPG
Packing\O2k-Box 2, for shipment of accessories.
Packing\Peli Case
Packing Peli Case.jpg
Packing\Peli Case: Watertight, crushproof, and dust proof case for safe transportation of the O2k:

Retractable extension handle, Strong polyurethane wheels with stainless steel bearings, Easy open Double Throw latches, Open cell core with solid wall design - strong, light weight, O-ring seal, Automatic Pressure Equalization Valve, Fold down handles, Stainless steel hardware and padlock protectors, 4 level Pick N Pluck™ with convoluted lid foam, Special foam inlets for O2k.

Interior Dimensions: 21.48" x 16.42" x 12.54" (54.5 x 41.7 x 31.8 cm)
Pipette\Plastic\1 ml ungraded
Pipette Plastic 1 ml-ungraded.JPG
Pipette\Plastic\1 mL ungraded, for filling electrolyte into the reservoir of the OroboPOS.
Plunger\10 mm3
Plunger 10 mm3.JPG
Plunger\10 mm3 for Microsyringe\10 mm3 51/0.13 mm, spare.
Power O2k Numbers
Power O2k Numbers.JPG
Power O2k Numbers: Single number to label the O2k in a Power O2k-Lab.
Power O2k-FluoRespirometer
Power O2k-FluoRespirometer
Power O2k-FluoRespirometer - optional configuration as additional system for increasing output combined with the O2k-FluoRespirometer (O2k-Series H). The Power O2k-FluoRespirometer includes the TIP2k and the O2k-sV-Module, and supports all add-on O2k-Modules of the Oroboros O2k. It can be added to an existing Oroboros O2k of any O2k-Series. This application does not require an additional ISS-Integrated Suction System and O2k-Titration Set. Furthermore, the OroboPOS-Mounting Tool of the OroboPOS Service Tools can be used from the available O2k and is not included.
Reference-Electrode\2.4 mm
Reference Electrode 2.4 mm.jpg
Reference-Electrode\2.4 mm: 2.4 mm diameter glass shaft, for ISE.
Replacement-Barrel
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Replacement-Barrel for Reference-Electrode\2.4 mm, 2.4 mm diameter glass.
Scissors\stainless steel\straight Tip\sharp
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Scissors\stainless steel\straight Tip\sharp.
Screwdriver allen wrench
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Screwdriver allen wrench, a standard component of the O2k-FluoRespirometer and O2k-sV-Module.
Shredder-Tube Ram Tool
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Shredder-Tube Ram Tool: component of PBI-Shredder O2k-Set.
Shredder-Tubes
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Shredder-Tubes: consisting of Shredder Tube FT500-PS with Lysis Disk, serrated Shredder-Tube Ram Tool and Shredder-Tube Cap Tool, coral colour (Box of 100) and Shredder Tube FT500-PMS with Lysis Disk, serrated Shredder-Tube Ram Tool and Shredder-Tube Cap Tool, coral colour (Box of 100). There is no difference between the white and the coral shredder tubes, except that the white tubes are also approved for high pressure.
Smart Fluo-Sensor Blue
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Smart Fluo-Sensor Blue: Excitation LED 465 nm (dominant wavelenght) with short pass filter, emission (red) with long pass filter, individual sensors are calibrated with sensor-specific memory and direct input into DatLab 7 to obtain reproducible light intensities with different sensors, including photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with safranin and rhodamine 123. Filter Set MgG / CaG for Magnesium green and Calcium green measurements are included.
Smart Fluo-Sensor Green
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Smart Fluo-Sensor Green: Excitation LED 525 nm (dominant wavelength) with short pass filter, emission (red) with long pass filter, individual sensors are calibrated with sensor-specific memory and direct input into DatLab 7 to obtain reproducible light intensities with different sensors, including photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed and TMRM measurements when delivered.
Stirrer-Bar sV\white PVDF\11.5x6.2 mm
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Stirrer-Bar sV\white PVDF\11.5x6.2 mm, operated in the 0.5-mL O2k-Chamber sV at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor (OroboPOS) placed in a position of maximum current of the medium.
Stirrer-Bar\black PEEK\15x6 mm
Stirrer-Bar black PEEK15x6 mm.JPG
Stirrer-Bar\black PEEK\15x6 mm, for 2-mL O2k-Chamber, for specific optical applications to minimize optical interference.
Stirrer-Bar\white PVDF\15x6 mm
Stirrer bar PVDF.jpg
Stirrer-Bar\white PVDF\15x6 mm, operated in the 2-mL O2k-chamber at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor (OroboPOS) placed in a position of maximum current of the medium.
Stopper sV\black PEEK\conical Shaft\central Port
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Stopper sV\black PEEK\conical Shaft\central Port: with conical shaft (with PTFE, graphite, carbon fiber) and one central capillary (1.0 mm diameter; 48.9 mm length), Volume-Calibration Ring sV (A or B) for volume adjustment 0.5 mL; 2 mounted O-rings (O-ring sV\Viton\9.5x1 mm).
Stopper-Needle
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Stopper-Needle: Short needle for bubble extrusion from port of the ISE-stopper.
Stopper-Spacer
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Stopper-Spacer (or gas spacer) to set the O2k-Stopper into a standard position for a fixed gas phase above the aqueous phase in the 2-mL O2k-chamber, during air calibration or for injection of nitrogen, argon or oxygen into the gas phase; Stopper-Spacer thickness of 4mm (a deviation of +/- 10% possible).
Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port
Stopper black PEEK angular Shaft side+6.2+2.6 mm Port.JPG
Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port, for application with ISE; side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm).
Stopper\black PEEK\conical Shaft\central Port
Stopper black PEEK conical Shaft central Port.JPG
Stopper\black PEEK\conical Shaft\central Port: with conical shaft (with PTFE, graphite, carbon fiber) and one central capillary (1.3 mm diameter; 50.6 mm length), Volume-Calibration Ring (A or B) for volume adjustment 1.5 to 3.2 ml; 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). The black PEEK stoppers are required for optical O2k-MultiSensor Modules.
Stopper\black PEEK\conical Shaft\central Port\peripheral 2.3 mm Port
Stopper black PEEK conical Shaft central Port peripheral 2.3 mm Port.JPG
Stopper\black PEEK\conical Shaft\central+peripheral 2.3 mm Port: for potentiometric O2k-MultiSensor applications (NO, H2S, H2O2); 1 additional port (2.3 mm); central titration port; conical bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm).
Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port
Stopper black PEEK conical Shaft central+2.3+2.6 mm Port.JPG
Stopper\black PEEK\conical Shaft\central+2.3+2.6 mm Port: for pH and reference electrode, central titration port and two additional ports (2.3 mm and 2.6 mm); conical bottom; including Volume-Calibration Ring (A or B), 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm).
Syringe Collars
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Syringe Collars (package of 20) for Hamilton microsyringes: for correctly storing the microsyringes in the Syringe Racks.
Syringe Labels
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Syringe Labels: set of labels with standard abbreviations (see O2k-Titrations).
Syringe Racks
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Syringe Racks: stainless steel; for proper placement of eight Hamilton microsyringes in HRR experiments; package of two (for a total of 16 microsyringes).
Syringe Storage Box
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Syringe Storage Box: for storing the Hamilton microsyringes; includes Syringe Labels.
Syringe\500 mm3 51/0.41 mm
Syringe 500 mm3 51 0.41 mm.JPG
Hamilton Syringe\500 mm3 51/0.41 mm for manual titrations, 500 mm3 volume; fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter; for injections of suspensions of isolated mitochondria and filling of the Microsyringe\200 mm3\TIP2k.
Syringe\60 ml\Gas-Injection
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Syringe\60 mL\Gas-Injection, 60 mL, with spacer and stainless steel needle, flat tip, for gas injection into the O2k-chamber.
TIP2k Filter Papers
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TIP2k Filter Papers (package of 10) to be used for the stoppers during TIP2k titration for Instrumental oxygen background correction to avoid constant manual siphoning off of extruded liquid.
TIP2k-Module
TIP2k with 200 mm3 microsyringe.JPG
TIP2k-Module - Titration-Injection microPump (TIP2k) for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat).
TIP2k-Needle Safety Support with cable guide
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TIP2k-Needle Safety Support with cable guide: for safe storage of TIP2k-needles and MultiSensor Modules cables when not required during the experiment.
TIP2k-Needle Spacer
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TIP2k-Needle Spacers for microinjection TIP2k needle, silicone stops (200/Pkg.) with mounting tool.
TestAbbrDescr
Titration-Injection microPump
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Titration-Injection microPump (TIP2k) for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control (oxystat, pH-stat).
Tube Racks
Tube Racks.JPG
Tube Racks: stainless steel, accomodating four 50-ml tubes, for cleaning of microsyringes, cleaning and storage of stoppers during cleaning of the O2k-chambers; package of two (for a total of eight tubes).
USB-Cable 2.0\Type A-B
USB-Cable2.0 Type A-B.JPG
USB-Cable 2.0\Type A-B for connecting the O2k-Main Unit (O2k-Series E upwards) to the USB port of a PC or laptop.
V-ring\30-35-4.5 mm
V-Ring 30-35-4.5.JPG
V-ring\30-35-4.5 mm, mounted on O2k-Chamber Holder and O2k-Chamber Holder sV.
Volume-Calibration Ring
Volume calibration ring A+B PVDF for Stopper.jpg
Volume-Calibration Ring (A or B) PVDF for O2k-Stoppers (2-mL O2k-chamber), white; attached to the stoppers.
Volume-Calibration Ring sV
Volume calibration ring A+B PVDF for Stopper.jpg
Volume-Calibration Ring sV (A or B), white PVDF, attached to PEEK O2k-Stopper sV.