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MitoPediaAlert2021 Archive

From Bioblast
Currently Answered Questions from the »Oroboros Ecosystem Agenda 2021 Archive«

Dec 2021

  • Dec-13 Mo
Why use a short-chain coenzyme Q mimetic for measurement of the Q-redox state using the Q-Module?

The naturally occurring long-isoprenoid chain coenzyme Q (e.g. CoQ10 or CoQ9) is trapped within membrane boundaries. Therefore, a short-chain, membrane-permeable CoQ mimetic, CoQ2 is used as a probe which reacts with the mitochondrial respiratory Complexes at their quinone-binding sites and with the working electrode of the Q-sensor.
- »Coenzmye Q2« and »Q-Module« 
- communicated by »Komlodi Timea« and »Schmitt Sabine« 

  • Dec-08 We
The central role of the Q-junction in mitochondria

The Q-junction is a point of convergence in the electron transfer system. Electrons flow from different dehydrogenases and respiratory Complexes into the Q-junction and then downstream to Complex III.
- »Q-junction« 
- communicated by »Komlodi Timea« and »Schmitt Sabine« 

  • Dec-02 Th
Coenzyme Q - a mobile redox compound in the electron transfer system

Coenzyme Q is a lipid composed of a benzoquinone ring with an isoprenoid side chain, two methoxy groups and one methyl group being present in all tissues and cells. In humans, CoQ10 occurring in the mitochondrial electron transfer system transfers electrons from dehydrogenases and respiratory complexes downstream to Complex III.
- »Coenzyme Q« 
- communicated by »Komlodi Timea« and »Schmitt Sabine« 

Nov 2021

  • Nov-24 We
Mitochondrial H2O2 production was discovered 50 years ago by the group of Britton Chance

Check out how to measure H2O2 production using the Amplex UltraRed assay in combination with high-resolution respirometry.
- »Loschen 1971 FEBS Letters« and »Amplex UltraRed« and »O2k-Fluo LED2-Module« »O2k-Fluo Smart-Module« 
- communicated by »Komlodi Timea« and »Schmitt Sabine« 

Jul 2021

  • Jul-07 We
TPP+ for potentiometric detection of mt-membrane potential (mtMP) or fluorometry with safranin?

Safranin enables measurement of the relationship between fluorescence intensity and mtMP, and if linear, the absolute mtMP can be calculated. TPP+ enables calibration for measurement of mtMP.
- »TPP+ vs safranin«

Jun 2021

  • Jun-24 Th
Do you know how to select the correct fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) enables measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«

May 2021

  • May-24 Mo
Immediately after switching off the light, a sharp peak of light-enhanced dark respiration is observed in algae.

- »Light-enhanced dark respiration«

  • May-11 Tu
MitoPedia: SUIT Measuring succinate pathway (S-pathway) in living cells

Using MitoKit-CII – cell permeable succinate and malonate, the S-pathway can be measured
- »SUIT-003 O2 ce D060«
- communicated by »Cecatto Cristiane« and »Komlodi Timea« 

Apr 2021

Mar 2021

  • Mar-16 Tu
CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration

Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5« 
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

  • Mar-11 Th
Gnaiger 2020 BEC MitoPathways Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?

A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3« 
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«

  • Mar-2 Tu
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate

In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5« 
- communicated by »Cecatto Cristiane« and »Cardoso Luiza« 

Jan 2021

  • Jan-21 Th
Measuring ATP production with Magnesium Green – which sample preparations work?

Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG« 
- communicated by »Cardoso Luiza« and »Komlodi Timea«