MitoPediaAlert2022 Archive

Currently Answered Questions from the »Oroboros Ecosystem Agenda 2022 Archive«

Oct 2022

Oct-28 Fr

BEC tutorial: Is the thermodynamics of irreversible processes based on an outdated paradigm?

MitoPedia term — MitoPedia: Ergodynamics — Linear phenomenological laws

Oct-21 Fr

BEC tutorial: How intensive is an intensive — in contrast to extensive — quantity?

MitoPedia term — Intensive quantity - such as force and pressure

Oct-18 Tu

BEC tutorial: Find out the straight (yet enigmatic) connection between Gibbs energy G [J] and the 'molar Gibbs energy' of a transformation [J·mol^-1], which is better called the Gibbs force:

MitoPedia term — Gibbs energy

Sep 2022

Sep-06 Tu

How does proficiency testing help achieve quality and reproducibility?

Providing a pre-established set of testing criteria improves and standardizes technical performance.
- »Proficiency test«
- communicated by »Baglivo Eleonora« and »Tindle-Solomon Lisa«

Aug 2022

Aug-15 Mo

BEC tutorial: Did you know that a cell respiring O₂ at 100 amol·s^-1·x^-1 consumes 60 million O₂ molecules per second per cell?

Apply the Avogadro constant and SI prefixes: 100 (amol O₂)·s^-1·(x cell)^-1 = 60.2·10⁶ (x O₂)·s^-1·(x cell)^-1

Aug-04 Th

Why do we perform multiple H₂O₂ calibrations in the Amplex® UltraRed assay?

The sensitivity to H₂O₂ of the fluorometric signal changes during the experiment as a function of: (1) experimental time and accumulating UltroxRed (similar to resorufin), (2) changes of the optical properties due to titrations, and (3) the radical scavenging capacity of the sample. Therefore, H₂O₂ calibrations are performed before and after sample addition, and after selected titration steps.

- »Amplex UltraRed« and »Komlodi et al BEC 2021.4«

Aug-02 Tu

What are the substrate-uncoupler-inhibitor titration reference protocols?

These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
- » SUIT reference protocol«

- communicated by »Cardoso Luiza HD« and »Tindle-Solomon Lisa«

Jul 2022

Jul-27 We

Body mass M and mass m - what is the difference? Consult MitoPedia for a comment on body mass versus body weight.

- »Body mass with a link to mitObesity«

Jul-25 Mo

Do scientists take responsibility for energy saving? - Or does biomedical research merely find excuses?

- »Energy saving in research« — more details: MitoPedia: Oroboros QM

Jul- 19 Tu

Do you want to find the best SUIT protocol for your research questions?

- Use the SUITbrowser: »SUITbrowser«

Jul- 18 Mo

What is research?

- »Research«

Jul- 13 We

"Assuming a publication charge of € 1200 per article, scientists mentioning mitochondria or photosynthesis pay € 100 000 every day in 2021 for ‘selling’ their output to publishers ― over € 36 Mill per year."

- Gnaiger E (2021) Beyond counting papers – a mission and vision for scientific publication. https://doi.org/10.26124/bec:2021-0005

- »Paywall journalism«

Jul- 07 Th

Take some time for history - it adds to your stories:

- »Mitochondria and bioblasts: Made history«

Jun 2022

Jun- 15 We

The central role of the Q-junction in mitochondria

The Q-junction is a point of convergence in the electron transfer system. Electrons flow from different dehydrogenases and respiratory Complexes into the Q-junction and then downstream to Complex III.
- »Q-junction«
- communicated by »Schmitt Sabine«

May 2022

May- 24 Tu

Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- »Setting the oxygen concentration«

Mar 2022

Mar- 18 Fr

Paywall journalism is the opposite of Open Access.

Though often defined from the individual reader's perspective, a paywall can also apply to an institution (such as a library) or the author.
- »Paywall journalism« , »Gnaiger (2021) Editorial Bioenerg Commun«