MitoPediaAlert2022 Archive

From Bioblast
Currently Answered Questions from the »Oroboros Ecosystem Agenda 2022 Archive«

Oct 2022

  • Oct-28 Fr
Gnaiger 2020 BEC MitoPathways
BEC tutorial: Is the thermodynamics of irreversible processes based on an outdated paradigm?

  • Oct-21 Fr
Gnaiger 2020 BEC MitoPathways
BEC tutorial: How intensive is an intensive — in contrast to extensive — quantity?

  • Oct-18 Tu
Gnaiger 2020 BEC MitoPathways
BEC tutorial: Find out the straight (yet enigmatic) connection between Gibbs energy G [J] and the 'molar Gibbs energy' of a transformation [J·mol-1], which is better called the Gibbs force:

Sep 2022

  • Sep-06 Tu
Oroboros quality management
How does proficiency testing help achieve quality and reproducibility?

Providing a pre-established set of testing criteria improves and standardizes technical performance.
- »Proficiency test«
- communicated by »Baglivo Eleonora« and »Tindle-Solomon Lisa«

Aug 2022

  • Aug-15 Mo
Gnaiger 2020 BEC MitoPathways
BEC tutorial: Did you know that a cell respiring O2 at 100 amol·s-1·x-1 consumes 60 million O2 molecules per second per cell?

Apply the Avogadro constant and SI prefixes: 100 (amol O2)·s-1·(x cell)-1 = 60.2·106 (x O2)·s-1·(x cell)-1

  • Aug-04 Th
Why do we perform multiple H2O2 calibrations in the Amplex® UltraRed assay?

The sensitivity to H2O2 of the fluorometric signal changes during the experiment as a function of: (1) experimental time and accumulating UltroxRed (similar to resorufin), (2) changes of the optical properties due to titrations, and (3) the radical scavenging capacity of the sample. Therefore, H2O2 calibrations are performed before and after sample addition, and after selected titration steps.

- »Amplex UltraRed« and  »Komlodi et al BEC 2021.4«

  • Aug-02 Tu
MitoPedia: SUIT What are the substrate-uncoupler-inhibitor titration reference protocols?

These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
- » SUIT reference protocol«

- communicated by »Cardoso Luiza HD« and »Tindle-Solomon Lisa«

Jul 2022

  • Jul-27 We
Body mass M and mass m - what is the difference? Consult MitoPedia for a comment on body mass versus body weight.

- »Body mass with a link to mitObesity« 

  • Jul-25 Mo
Oroboros quality management
Do scientists take responsibility for energy saving? - Or does biomedical research merely find excuses?

- »Energy saving in research« — more details: MitoPedia: Oroboros QM

  • Jul- 19 Tu
MitoPedia: SUIT Do you want to find the best SUIT protocol for your research questions?

- Use the SUITbrowser: »SUITbrowser«

  • Jul- 18 Mo
Oroboros quality management
What is research?

- »Research« 

  • Jul- 13 We
Graphical abstract
"Assuming a publication charge of € 1200 per article, scientists mentioning mitochondria or photosynthesis pay € 100 000 every day in 2021 for ‘selling’ their output to publishers ― over € 36 Mill per year."
- Gnaiger E (2021) Beyond counting papers – a mission and vision for scientific publication.
- »Paywall journalism« 

  • Jul- 07 Th
Mitochondria and bioblasts: Made history
Take some time for history - it adds to your stories:

- »Mitochondria and bioblasts: Made history« 

Jun 2022

  • Jun- 15 We
The central role of the Q-junction in mitochondria

The Q-junction is a point of convergence in the electron transfer system. Electrons flow from different dehydrogenases and respiratory Complexes into the Q-junction and then downstream to Complex III.
- »Q-junction« 
- communicated by »Schmitt Sabine« 

May 2022

  • May- 24 Tu
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- »Setting the oxygen concentration«

Mar 2022

  • Mar- 18 Fr
Paywall journalism is the opposite of Open Access.

Though often defined from the individual reader's perspective, a paywall can also apply to an institution (such as a library) or the author.
- »Paywall journalism« , »Gnaiger (2021) Editorial Bioenerg Commun«

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