Mitochondrial preparations

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Mitochondrial preparations


Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the cell membranes are either removed (imt and smtp) or mechanically (thom) and chemically permeabilized (pfi), while the mitochondrial functional integrity and to a large extent the mt-structure is maintained. According to this definition, submitochondrial particles (smtp) are not a mtprep.

Abbreviation: mtprep

MitoPedia topics: Sample preparation 

Communicated by Gnaiger E 2011-11-15, edited 2017-04-20.

MitoPedia: mt-preparations

» Compare: Submitochondrial particles, smtp
O2k-Protocols for mt-preparations: O2k-mitochondrial preparations


Bioblast linkReferenceYear
Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.2019
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1.2020


Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate