Moll 2016 bioRxiv
|Moll CWI, Lindtner RA, Vogel GF, Brunner M, Gutleben K, Blauth M, Krumschnabel G, Ebner HL (2016) Bone marrow mesenchymal stem cells from osteoporotic patients do not show altered mitochondrial energetics or ultrastructure. bioRxiv doi.org/10.1101/046300.|
Abstract: As a consequence of the ongoing demographic change, osteoporosis is considered as one of the mayor challenges for the health care system of the 21st century. However, the exact etiology of osteoporosis is far from being understood. Some evidence suggests that changes in stem cell metabolism might contribute to development of the disease. Therefore we evaluated whether differences of the morphology and/or the energy metabolism of mitochondria can be observed between human bone marrow derived mesenchymal stem cells obtained from osteoporotic patients as compared to non-osteoporotic controls. Mesenchymal stem cells were isolated from the bone marrow of senile osteoporotic and non osteoporotic patients, osteoporosis being assessed by dual energy X-ray absorptiometry. We then confirmed the stemness of the cells by FACS analysis of the expression of surface markers and by conducting multi-lineage differentiation experiments. And we finally investigated mitochondrial morphology and function with electron microscopy of cryo-fixed samples and by high-resolution respirometry, respectively. In addition we compared the energy metabolism of the stem cells to those of the osteosarcoma cell line MG-63. The data show, for the first time, the applicability on stem cells of the methods used here. Furthermore, our results indicated that there are no obvious differences detectable in mitochondrial morphology between cells from osteoporotic and non osteoporotic donors and that these cells also seem to be energetically indistinguishable with unchanged rates of ROUTINE respiration and respiratory capacity as well as unaltered oxygen consumption rates linked to different respiratory complexes. In summary, we could not detect any evidence indicating major changes of mitochondrial features in cells from osteoporotic patients.
• Keywords: Osteoporosis, Mitochondria, Stem cell metabolism, Human bone marrow derived mesenchymal stem cell differentiation, High-resolution respirometry, Electron microscopy • Bioblast editor: Krumschnabel G • O2k-Network Lab: AT Innsbruck Oroboros
Labels: MiParea: Respiration Pathology: Aging;senescence, Other
Organism: Human Tissue;cell: Stem cells Preparation: Intact cells, Permeabilized cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, S, ROX HRR: Oxygraph-2k