Nabben 2008 FEBS Lett

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Nabben M, Hoeks J, Briedé JJ, Glatz JF, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) The effect of UCP3 overexpression on mitochondrial ROS production in skeletal muscle of young versus aged mice. FEBS Lett 582:4147-52.

» PMID: 19041310 Open Access

Nabben M, Hoeks J, Briede JJ, Glatz JF, Moonen-Kornips E, Hesselink MK, Schrauwen P (2008) FEBS Lett

Abstract: Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance spectroscopy, but only after addition of 4-hydroxynonenal (4-HNE). Mitochondrial function, assessed by respirometry, on glycolytic substrate was lower in UCP3Tg mice compared to wild types, whereas this tended to be higher on fatty acids. State 4o respiration was higher in UCP3Tg animals. To conclude, UCP3 overexpression leads to increased state 4o respiration and, in presence of 4-HNE, blunts the age-induced increase in ROS production.

Keywords: UCP3, Uncoupling, ROS, Lipotoxicity, Mitochondria, High resolution respirometry, 4-HNE

O2k-Network Lab: NL Maastricht Schrauwen P


Labels: MiParea: Respiration, Genetic knockout;overexpression  Pathology: Aging;senescence, Diabetes  Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria  Enzyme: Uncoupling protein  Regulation: ADP, Substrate, Uncoupler, Fatty acid  Coupling state: OXPHOS 

HRR: Oxygraph-2k