Naimi 2011 Clin Physiol Funct Imaging
|Naimi AI, Bourbeau J, Perrault H, Baril J, Wright-Paradis C, Rossi A, Taivassalo T, Sheel AW, Rabøl R, Dela F, Boushel RC (2011) Altered mitochondrial regulation in quadriceps muscles of patients with COPD. Clin Physiol Funct Imaging 31:124-31.|
Abstract: Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine State 2 respiration was higher in COPD; (ii) State 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to Complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s(-1) mg tissue(-1) ), but O(2) flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s(-1) mg tissue(-1) ); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.
• Keywords: chronic obstructive pulmonary disease, mitochondrial respiration, skeletal muscle
Labels: Pathology: COPD Stress:Mitochondrial disease Organism: Human Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue Enzyme: Complex I, Complex IV;cytochrome c oxidase
Coupling state: OXPHOS