NextGen-O2k Documentation

From Bioblast
Jump to navigation Jump to search

Bioblast 2022

NextGen-O2k Documentation


The revolutionary all-in-one instrument to conquer mitochondrial disease.

WP3: Documentation


In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.


Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published.

Progress and next steps

See » Track record

PlayVideo.jpg NextGen-O2k Videosupport clips

Click to expand or collaps
Click to expand or collaps
Click to expand or collaps
Click to expand or collaps
Click to expand or collaps
Click to expand or collaps

NextGen-O2k demo

  • NextGen-O2k demo: Coenzyme Q redox state and mt-respiration
  • NextGen-O2k demo: mt-respiration and NADH
  • NextGen-O2k demo: mt-respiration and H2O2
  • NextGen-O2k demo: H2O2 calibration

O2k-Manual.jpg NextGen-O2k Manuals

» NextGen-O2k manual
» DatLab8.0: CV-Module
» NextGen-O2k: Q-Module
» NextGen-O2k: NADH-Module
» NextGen-O2k: PB-Module
» O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8
» Oxygen flux_analysis - DatLab 7.4
» Safranin Analysis Template
» General template for mt-membrane potential analysis
» H2O2 flux analysis