NextGen-O2k Scientific developments

From Bioblast
Jump to navigation Jump to search

Bioblast 2022



NextGen-O2k Scientific developments

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP2: Scientific developments



Abstract

The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
  • Determination of optimal Q-sensor measurement conditions
  • Determination of optimal measurement conditions for the PB-Module
  • Development of protocols for scientific, pre-clinical and algal biotechnology applications
  • Data handling and quality control

Achievements

Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.
Project Year 2: During the second project year, measurement conditions and protocols were optimized for the PB- and Q-Modules with a variety of biological samples. Additionally, the NADH-Module for evaluation of the NADH-pool in physio-pathological conditions was integrated in the NextGen-O2k. Testing of modules for the DatLab8 software were conducted and improvements applied. Moreover, SUIT protocols for general, pre-clinical, and algal biotechnology applications were developed. Quality control was maintained during the second period of project, an important factor in the development of the NexGen-O2k instrument and relevant protocols.

Progress and next steps

See » Track record

NextGen-O2k MitoFit Preprints

LinkViewReferenceYear
MitoFit Preprints 2022.19.
MitoFit pdf
The robustness of NextGen-O2k for building PI curves in microalgae »Watch the presentation«
Vera-Vives AM, Perin G, Morosinotto T (2022) The robustness of NextGen-O2k for building PI curves in microalgae. https://doi.org/10.26124/mitofit:2022-00192022-05-12
MitoFit Preprints 2022.1.v3.
MitoFit pdf
Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I
Pallag G, Nazarian S, Ravasz D, Bui D, Komlódi T, Doerrier C, Gnaiger E, Seyfried TN, Chinopoulos C (2022) Proline oxidation leading to high electron flow through reduction of ubiquinone supports ATP production by F1FO-ATPase in mitochondria with inhibited Complex I. https://doi.org/10.26124/mitofit:2022-0001.v3 - 2022-05-04 published in https://doi.org/10.3390/ijms230951112022-03-07
MitoFit Preprints 2021.5.
MitoFit pdf
Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry
Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. https://doi.org/10.26124/mitofit:2021-00052021-04-30
MitoFit Preprint Arch EA20.
MitoFit pdf
High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations
Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.00012020-11-30