NextGen-O2k Scientific developments

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NextGen-O2k Scientific developments

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP2: Scientific developments



Abstract

The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
  • Determination of optimal Q-sensor measurement conditions
  • Determination of optimal measurement conditions for the PB-Module
  • Development of protocols for scientific, pre-clinical and algal biotechnology applications
  • Data handling and quality control

Achievements

Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.

Progress and next steps

2020-10-08: Laurent Beck started with measurements on Chlamydomonas reinhardtii using the PB- and Q-Modules , supervised by PI Maria Huete-Ortega and Thomas Roach (University of Innsbruck, Institute of Botany)
2020-07-23: First isolation of mitochondria in microalgae trials
2020-07-01: Start of the development of protocols for permeabilizing microalgae cells
2020-04-24: First tests with pH electrodes
2020-03-11: First tests with Calcium Green
2020-02-20: Started development of protocols (and DL-Protocols) for the PB-Module, including new SUIT protocols for photosynthesis measurements.
2019-12-31: Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
2019-12-06: First version of the Cyclic Voltammetry Manual released: CV-Module
2019-12-02: Optimization of O2k-Procedures for isolated mitochondria preparations
2019-11-22: First version of NextGen-O2k Manual/SOP finalized: NextGen-O2k: Q-Module
2019-11-21: Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
2019-11-18: Application of Q-redox state measurements in scientific projects
2019-10-22: 66 SUIT protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see MitoPedia:_SUIT
2019-10-21: Four new videos for DatLab 7.4 software released - see: O2k-Videosupport DatLab 7.4
2019-09-24: Two new videos for mitochondrial preparations released - see: O2k-Videosupport Mitochondrial preparations
2019-09-23: First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1st Q-Module workshop at the Oroboros O2k-Workshop IOC141
2019-09-20: First six videos for the Q-Module released - see: O2k-Videosupport Q-Module
2019-09-06: Development of two harmonized SUIT protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
2019-07-17: First tests of the new O2 analysis template DL7.4
2019-07: Started development of SUIT protocols for isolated mitochondria and permeabilzied cells
2019-06-18: Testing of the SUITbrowser by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139
2019-06: Integration of cyclic voltammetry as a quality control step into the Q-Module
2019-06: Testing of the SUITbrowser

Links and references