O2k-Demo experiments

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OROBOROS support
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O2k-Demo experiments

O2k-Procedures
SUIT-Protocol

Oroboros O2k demo

ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Gnaiger 2020 BEC MitoPathwaysBioblast pdfPublished online: 2020-Dec-30

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2: 112 pp. doi:10.26124/bec:2020-0002.Permeabilized cells
Permeabilized tissue
Homogenate
Isolated mitochondria
Intact cells
Heart
Skeletal muscle
Fibroblast
Human
Mouse
2020
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
Permeabilized tissueSkeletal muscleMouse2018-11-18
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
Intact cellsBlood cells
Lymphocyte
Human2016-08-11
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
HomogenateHeart
Liver
Fishes2015-06-18
MiPNet12.11 MitoRespirationBioblast pdf »Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
Isolated mitochondriaArtemia
Crustaceans
2014-02-20
Pesta 2012 Methods Mol BiolPMID: 22057559
Bioblast pdf
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.Permeabilized cells
Permeabilized tissue
Intact cells
Skeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Human2012
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.Permeabilized cells
Permeabilized tissue
Intact cells
Blood cellsMouse2008
TIP2k-Module

O2k-Demo TIP2k

 » O2k-Catalogue: TIP2k
 » O2k-Publications: TIP2k
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
Pesta 2012 Methods Mol BiolPMID: 22057559
Bioblast pdf
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58.Permeabilized cells
Permeabilized tissue
Intact cells
Skeletal muscle
Other cell lines
HEK
Fibroblast
HUVEC
Human2012
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.Permeabilized cells
Permeabilized tissue
Intact cells
Blood cellsMouse2008
MiPNet04.05 Titration-InjectionBioblast pdf
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
Intact cellsEndothelial;epithelial;mesothelial cell
HUVEC
Human1999-01

TIP2k Manual

ChapterViewSectionLast update
MiPNet12.10 TIP2k-manualBioblast pdf »Versions
O2k-Manual
Titration-Injection microPump. TIP2k user manual.
2016-08-10



O2k-FluoRespirometer
link=O2k-TPP%2B_and_Ca2%2B_ISE-Module

O2k-Demo O2k-MultiSensor

 » O2k-Catalogue: O2k-MultiSensor
 » O2k-Publications: O2k-Fluorometry
ProtocolViewTopicPreparationTissue and cellMammal and modelLast update
MiPNet24.11 mtMP calculationPDF.jpg » Versions
O2k-Protocols
Mitochondrial membrane potential calculation
2019-08-05
MiPNet20.14 AmplexRed H2O2-productionBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
Isolated mitochondriaHeartMouse2019-06-24
MiPNet20.13 Safranin mt-membranepotentialBioblast pdf »Versions
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
HomogenateNervous systemMouse2019-06-24
MiPNet18.05 Amplex-Mouse-heartBioblast pdf »Versions
O2k-Protocols
O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate.
HomogenateHeartMouse2019-06-24
Krumschnabel 2015 Methods Mol BiolPMID: 25631019
O2k-Protocols contents
Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
Isolated mitochondriaNervous systemMouse2015
Makrecka-Kuka 2015 BiomoleculesPMID: 26131977 Open Access
O2k-Protocols contents
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.Permeabilized cells
Homogenate
Isolated mitochondria
Intact cells
Heart
Nervous system
HEK
Human
Mouse
2015
Krumschnabel 2014 Methods EnzymolPMID: 24862266 »O2k-brief
O2k-Protocols contents
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.HomogenateNervous systemMouse2014
MiPNet08.12 IOC22Bioblast pdf
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
Isolated mitochondriaLiverRat2011-12-11

O2k-MultiSensor Manual

ChapterViewSectionLast update
MiPNet15.03 O2k-MultiSensor-ISEBioblast pdf »Versions
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2018-01-25
MiPNet15.05 NO-manualBioblast pdf  »Versions
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-ModuleBioblast pdf »Versions
O2k-Manual
O2k-Fluo LED2-Module.
2016-08-09
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08


O2k-Guides: »O2k-Catalogue :: »O2k-Manual :: »O2k-Publications

O2k-Procedures » Link
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» O2k-Procedures: MitoPathways
SUIT-Protocol
» O2k-Demo experiments
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» O2k-chemicals and media
PBI-Shredder HRR-Set.JPG
» O2k-mitochondrial preparations
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» O2k-Procedures: Mitochondrial and marker-enzymes